Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

David Wright; Zoltan Ferjentsik; Shang-Wei Chong; Xuehui Qiu; Yun-Jin Jiang; Pascale Malapert; Olivier Pourquie; Nick Van Hateren; Stuart A. Wilson; Claudio Franco; Holger Gerhardt; J. Kim Dale; Miguel Maroto

doi:10.1002/dvdy.22139

Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

David Wright, Zoltan Ferjentsik, Shang-Wei Chong, Xuehui Qiu, Yun-Jin Jiang, Pascale Malapert, Olivier Pourquie, Nick Van Hateren, Stuart A. Wilson, Claudio Franco, Holger Gerhardt, J. Kim Dale, Miguel Maroto

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes. Developmental Dynamics 238:3043-3055, 2009. (C) 2009 Wiley-Liss, Inc.

Original language	English
Pages (from-to)	3043-3055
Number of pages	13
Journal	Developmental Dynamics
Volume	238
Issue number	12
DOIs	https://doi.org/10.1002/dvdy.22139
Publication status	Published - Dec 2009

Keywords

Nrarp
Notch pathway
Embryo
Somitic oscillator
PSM
Cyclic gene
Notch signaling pathway
Presomitic mesoderm
Segmentation clock
Primitive streak
Gene expression
Chick embryo
Zebrafish somitogenesis
Differentiation
Cells
Fate

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10.1002/dvdy.22139

1 Finished

Aref#d: 21932. The Interplay of Wnt and Notch Signalling in the Segmentation Clock Mechanism
Dale, K.
Wellcome Trust
23/11/09 → 22/11/13
Project: Research

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Wright, D., Ferjentsik, Z., Chong, S-W., Qiu, X., Jiang, Y-J., Malapert, P., Pourquie, O., Van Hateren, N., Wilson, S. A., Franco, C., Gerhardt, H., Dale, J. K., & Maroto, M. (2009). Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate. Developmental Dynamics, 238(12), 3043-3055. https://doi.org/10.1002/dvdy.22139

Wright, David ; Ferjentsik, Zoltan ; Chong, Shang-Wei ; Qiu, Xuehui ; Jiang, Yun-Jin ; Malapert, Pascale ; Pourquie, Olivier ; Van Hateren, Nick ; Wilson, Stuart A. ; Franco, Claudio ; Gerhardt, Holger ; Dale, J. Kim ; Maroto, Miguel. / Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate. In: Developmental Dynamics. 2009 ; Vol. 238, No. 12. pp. 3043-3055.

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title = "Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate",

abstract = "Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes. Developmental Dynamics 238:3043-3055, 2009. (C) 2009 Wiley-Liss, Inc.",

keywords = "Nrarp, Notch pathway, Embryo, Somitic oscillator, PSM, Cyclic gene, Notch signaling pathway, Presomitic mesoderm , Segmentation clock, Primitive streak, Gene expression, Chick embryo, Zebrafish somitogenesis, Differentiation, Cells, Fate",

author = "David Wright and Zoltan Ferjentsik and Shang-Wei Chong and Xuehui Qiu and Yun-Jin Jiang and Pascale Malapert and Olivier Pourquie and {Van Hateren}, Nick and Wilson, {Stuart A.} and Claudio Franco and Holger Gerhardt and Dale, {J. Kim} and Miguel Maroto",

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doi = "10.1002/dvdy.22139",

language = "English",

volume = "238",

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Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate. / Wright, David; Ferjentsik, Zoltan; Chong, Shang-Wei; Qiu, Xuehui; Jiang, Yun-Jin; Malapert, Pascale; Pourquie, Olivier; Van Hateren, Nick; Wilson, Stuart A.; Franco, Claudio; Gerhardt, Holger; Dale, J. Kim; Maroto, Miguel.

In: Developmental Dynamics, Vol. 238, No. 12, 12.2009, p. 3043-3055.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

AU - Wright, David

AU - Ferjentsik, Zoltan

AU - Chong, Shang-Wei

AU - Qiu, Xuehui

AU - Jiang, Yun-Jin

AU - Malapert, Pascale

AU - Pourquie, Olivier

AU - Van Hateren, Nick

AU - Wilson, Stuart A.

AU - Franco, Claudio

AU - Gerhardt, Holger

AU - Dale, J. Kim

AU - Maroto, Miguel

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AB - Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes. Developmental Dynamics 238:3043-3055, 2009. (C) 2009 Wiley-Liss, Inc.

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KW - Somitic oscillator

KW - PSM

KW - Cyclic gene

KW - Notch signaling pathway

KW - Presomitic mesoderm

KW - Segmentation clock

KW - Primitive streak

KW - Gene expression

KW - Chick embryo

KW - Zebrafish somitogenesis

KW - Differentiation

KW - Cells

KW - Fate

U2 - 10.1002/dvdy.22139

DO - 10.1002/dvdy.22139

M3 - Article

VL - 238

SP - 3043

EP - 3055

JO - Developmental Dynamics

JF - Developmental Dynamics

SN - 1058-8388

IS - 12

ER -

Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

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Keywords

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Aref#d: 21932. The Interplay of Wnt and Notch Signalling in the Segmentation Clock Mechanism

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