Cytochrome P-450 mediated genetic activity and cytotoxicity of seven halogenated aliphatic hydrocarbons in Saccharomyces cerevisiae

David F. Callen, C. Roland Wolf, Richard M. Philpot

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    90 Citations (Scopus)

    Abstract

    Cells of Saccharomyces cerevisiae, harvested from log-phase cultures, contain cytochrome P-450 and are capable of metabolizing promutagens to genetically active products. The activities of 7 halogenated aliphatic hydrocarbons in the yeast system have been investigated. All of the compounds tested (methylene chloride, halothane, chloroform, carbon tetrachloride, trichloroethylene, tetrachloroethylene and s-tetrachloroethane) induced mitotic gene convertants and recombinants and, to a lesser extent, gene revertants when incubated with logphase cells of the yeast strain D7. An examination of the difference spectra observed upon the addition of carbon tetrachloride, halothane and trichloroethylene to whole-cell or microsomal suspensions of yeast suggested that cytochrome P-450 mediated the metabolism of the hydrocarbons tested to cytotoxic and genetically active compounds.

    Original languageEnglish
    Pages (from-to)55-63
    Number of pages9
    JournalMutation Research/Genetic Toxicology
    Volume77
    Issue number1
    DOIs
    Publication statusPublished - 1 Jan 1980

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