Cytoplasmic lattices are not linked to mouse 2-cell embryos developmental arrest

Marianna Longo, Michele Boiani, Carlo Alberto Redi, Manuela Monti (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
112 Downloads (Pure)

Abstract

Cytoplasmic lattices are important regulators of oocyte maturation. They store components of the protein synthesis machinery including ribosomes and, among others, they are involved in the regulation of microtubule dynamics in both mouse and human. Cytoplasmic lattices undergo dramatic reorganizations at crucial stages of oocyte maturation, where they are abundantly present in the cytoplasm of developmentally competent oocytes named SN (Surrounded Nucleolus) while they are rare in the cytoplasm of 2-cell stage-arresting NSN (Not Surrounded Nucleolus) oocytes, suggestive of a requirement of cytoplasmic lattices for development past the 2-cell stage. Here, to elucidate this requirement, 2-cell mouse embryos derived from SN and NSN oocytes were analyzed by transmission electron microscopy. Contrary to what had been proposed hitherto, cytoplasmic lattices are present in 2-cell embryos derived not only from SN, but also from NSN oocytes, irrespective of the embryo production system (intra cytoplasmic sperm injection, parthenogenesis). Hence our conclusion that cytoplasmic lattices do not count among the factor(s) responsible for the embryo arrest at this crucial stage of development.
Original languageEnglish
Pages (from-to)267-270
Number of pages4
JournalEuropean Journal of Histochemistry
Volume62
Issue number4
Early online date24 Sep 2018
DOIs
Publication statusPublished - 24 Sep 2018

Keywords

  • Oocytes
  • cytoplasmic lattices
  • transmission electron microscopy
  • 2-cell embryo, developmental competence
  • ICSI
  • parthenogenesis

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