Cytotoxicity, genotoxicity and oxidative reactions in cell-culture models: modulatory effects of phytochemicals

N. M. O'Brien, J. A. Woods, S. A. Aherne, Y. C. O'Callaghan

    Research output: Contribution to journalArticlepeer-review

    67 Citations (Scopus)

    Abstract

    Much research effort has focused on the identification of phytochemicals in fruit and vegetables which exert beneficial effects. Our research examines modulatory effects of phytochemicals on cytotoxicity, genotoxicity and oxidative reactions in cell systems. Two examples of our studies are discussed. First, the potential beneficial effects of flavonoids are demonstrated. Flavonoids are reported to exhibit a wide variety of biological effects, including antioxidant and free-radical-scavenging activities. The aim of the study was to determine if flavonoids could protect against H2O2-induced DNA damage, as measured by the comet assay, in Caco-2 and HepG2 cells. Both cell lines were supplemented with increasing concentrations of myricetin, quercetin and rutin for 24 h followed by exposure to H2O2 (50 microM) for 30 min. Exposure to H2O2 for 30 min at 37 degrees C resulted in significant DNA damage and pre-incubation with the flavonoids before H2O2 exposure significantly (P
    Original languageEnglish
    Pages (from-to)22-26
    Number of pages5
    JournalBiochemical Society Transactions
    Volume28
    Issue number2
    DOIs
    Publication statusPublished - 2000
    Event670th Meeting of the Biochemical Society - Cork, Ireland
    Duration: 7 Sep 19999 Sep 1999

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