Cytotoxicity of xenobiotics and expression of glutathione-S-transferases in immortalised rat hepatocyte cell lines

K. Anderson, R. Andrews, L. Yin, R. McLeod, C. MacDonald, J. D. Hayes, M. H. Grant

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1. Immortalised rat hepatocyte cell lines are more sensitive to the cytotoxicity of 1-chloro-2,4-dinitrobenzene and ethacrynic acid than primary cultures of hepatocytes.

2. Class alpha glutathione S-transferases are not expressed in immortalised hepatocyte cell lines. Class pi glutathione S-transferase expression is elevated in the immortalised cell lines compared with freshly isolated hepatocytes, but it is not as high as in the HTC rat hepatoma cell line.

3. Immortalised hepatocyte cell lines may provide a sensitive model system for detecting cytotoxicity associated with xenobiotics which are detoxified by glutathione S-transferases.

Original languageEnglish
Pages (from-to)131-7
Number of pages7
JournalHuman & Experimental Toxicology
Volume17
Issue number3
DOIs
Publication statusPublished - Mar 1998

Keywords

  • Animals
  • Carcinoma, Hepatocellular
  • Cell Line
  • Cell Survival/drug effects
  • Dinitrochlorobenzene/toxicity
  • Ethacrynic Acid/toxicity
  • Glutathione Transferase/biosynthesis
  • Immunoblotting
  • Isoenzymes/biosynthesis
  • Liver/cytology
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Tetrazolium Salts
  • Thiazoles
  • Toxicity Tests
  • Xenobiotics/toxicity

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