Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate

Waheed Awotoye, Peter A. Mossey, Jacqueline B. Hetmanski, Lord J. J. Gowans, Mekonen A. Eshete, Wasiu L. Adeyemo, Azeez Alade, Erliang Zeng, Olawale Adamson, Olutayo James, Azeez Fashina, Modupe O. Ogunlewe, Thirona Naicker, Chinyere Adeleke, Tamara Busch, Mary Li, Aline Petrin, Abimbola Oladayo, Sami Kayali, Joy OlotuVeronica Sule, Mohaned Hassan, John Pape, Emmanuel T. Aladenika, Peter Donkor, Fareed K. N. Arthur, Solomon Obiri-Yeboah, Daniel K. Sabbah, Pius Agbenorku, Debashree Ray, Gyikua Plange-Rhule, Alexander Acheampong Oti, Daniah Albokhari, Nara Sobreira, Martine Dunnwald, Terri H. Beaty, Margaret Taub, Mary L. Marazita, Adebowale A. Adeyemo, Jeffrey C. Murray, Azeez Butali (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

4 Downloads (Pure)

Abstract

Objectives : Novel or rare damaging mutations have been implicated in the developmental pathogenesis of nonsyndromic cleft lip with or without cleft palate (nsCL ± P). Thus, we investigated the human genome for high-impact mutations that could explain the risk of nsCL ± P in our cohorts.

Materials and Methods: We conducted next-generation sequencing (NGS) analysis of 130 nsCL ± P case-parent African trios to identify pathogenic variants that contribute to the risk of clefting. We replicated this analysis using whole-exome sequence data from a Brazilian nsCL ± P cohort. Computational analyses were then used to predict the mechanism by which these variants could result in increased risks for nsCL ± P. Results : We discovered damaging mutations within the AFDN gene, a cell adhesion molecule (CAMs) that was previously shown to contribute to cleft palate in mice. These mutations include p.Met1164Ile, p.Thr453Asn, p.Pro1638Ala, p.Arg669Gln, p.Ala1717Val, and p.Arg1596His. We also discovered a novel splicing p.Leu1588Leu mutation in this protein. Computational analysis suggests that these amino acid changes affect the interactions with other cleft-associated genes including nectins (PVRL1, PVRL2, PVRL3, and PVRL4) CDH1, CTNNA1, and CTNND1.

Conclusion: This is the first report on the contribution of AFDN to the risk for nsCL ± P in humans. AFDN encodes AFADIN, an important CAM that forms calcium-independent complexes with nectins 1 and 4 (encoded by the genes PVRL1 and PVRL4). This discovery shows the power of NGS analysis of multiethnic cleft samples in combination with a computational approach in the understanding of the pathogenesis of nsCL ± P.

Original languageEnglish
Number of pages9
JournalCleft Palate-Craniofacial Journal
Early online date16 Nov 2022
DOIs
Publication statusE-pub ahead of print - 16 Nov 2022

Keywords

  • AFDN
  • cell adhesion molecule
  • cleft
  • craniofacial genetics
  • damaging mutations
  • next-generation sequencing
  • pathogenic variants
  • thermodynamics

Fingerprint

Dive into the research topics of 'Damaging Mutations in AFDN Contribute to Risk of Nonsyndromic Cleft Lip With or Without Cleft Palate'. Together they form a unique fingerprint.

Cite this