Day length dependent restructuring of the leaf transcriptome and metabolome in potato genotypes with contrasting tuberisation phenotypes

TY - JOUR

T1 - Day length dependent restructuring of the leaf transcriptome and metabolome in potato genotypes with contrasting tuberisation phenotypes

AU - Morris, Wayne L.

AU - Hancock, Robert D.

AU - Ducreux, Laurence J. M.

AU - Morris, Jenny A.

AU - Usman, Muhammad

AU - Verrall, Susan R.

AU - Sharma, Sanjeev K.

AU - Bryan, Glenn

AU - McNicol, James W.

AU - Hedley, Pete E.

AU - Taylor, Mark A.

N1 - This article is protected by copyright. All rights reserved.

PY - 2013/6

Y1 - 2013/6

N2 - Recent advances have defined some of the components of photoperiodic signalling that lead to tuberization in potato including orthologues of FLOWERING LOCUS T (StSP6A) and CYCLING DOF FACTOR (StCDF1). The aim of the current study is to investigate the molecular basis of permissive tuber initiation under long days in Solanum tuberosum Neo-Tuberosum by comparative analysis with an obligate short-day S.?tuberosum ssp. Andigena accession. We show that the Neo-Tuberosum accession, but not the Andigena, contains alleles that encode StCDF1 proteins modified in the C-terminal region, likely to evade long day inhibition of StSP6A expression. We also identify an allele of StSP6A from the Neo-Tuberosum accession, absent in the Andigena, which is expressed under long days. Other leaf transcripts and metabolites that show different abundances in tuberizing and non-tuberizing samples were identified adding detail to tuberization-associated processes. Overall, the data presented in this study highlight the subtle interplay between components of the clock-CONSTANS-StSP6A axis which collectively may interact to fine-tune the timing of tuberization. © 2013 John Wiley & Sons Ltd

AB - Recent advances have defined some of the components of photoperiodic signalling that lead to tuberization in potato including orthologues of FLOWERING LOCUS T (StSP6A) and CYCLING DOF FACTOR (StCDF1). The aim of the current study is to investigate the molecular basis of permissive tuber initiation under long days in Solanum tuberosum Neo-Tuberosum by comparative analysis with an obligate short-day S.?tuberosum ssp. Andigena accession. We show that the Neo-Tuberosum accession, but not the Andigena, contains alleles that encode StCDF1 proteins modified in the C-terminal region, likely to evade long day inhibition of StSP6A expression. We also identify an allele of StSP6A from the Neo-Tuberosum accession, absent in the Andigena, which is expressed under long days. Other leaf transcripts and metabolites that show different abundances in tuberizing and non-tuberizing samples were identified adding detail to tuberization-associated processes. Overall, the data presented in this study highlight the subtle interplay between components of the clock-CONSTANS-StSP6A axis which collectively may interact to fine-tune the timing of tuberization. © 2013 John Wiley & Sons Ltd

U2 - 10.1111/pce.12238

DO - 10.1111/pce.12238

M3 - Article

C2 - 24236539

SN - 1365-3040

VL - 37

SP - 1351

EP - 1363

JO - Plant, Cell & Environment

JF - Plant, Cell & Environment

IS - 6

ER -