DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA

Alban Ordureau, Karine Enesa, Sambit Nanda, Brice Le Francois, Mark Peggie, Alan Prescott, Paul R. Albert, Philip Cohen

    Research output: Contribution to journalArticle

    16 Citations (Scopus)
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    Abstract

    Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation-associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1)-dependent phosphorylation and activation of Interferon Regulatory Factor 3 (IRF3) and the E3 ubiquitin ligase Pellino1, which are required for interferon ß (IFNß) gene transcription. Here, we report that Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). The interaction is independent of the E3 ligase activity of Pellino1, but weakened by the phosphorylation of Pellino1. We show that DEAF1 binds to the IFNß promoter and to IRF3 and IRF7, that it is required for the transcription of the IFNß gene and IFNß secretion in MEFs infected with Sendai virus or transfected with poly(I:C). DEAF1 is also needed for TLR3-dependent IFNß production. Taken together, our results identify DEAF1 as a novel component of the signal transduction network by which dsRNA of viral origin stimulates IFNß production.
    Original languageEnglish
    Pages (from-to)24569-24580
    Number of pages12
    JournalJournal of Biological Chemistry
    Volume288
    Issue number34
    DOIs
    Publication statusPublished - 23 Aug 2013

    Fingerprint

    Sendai virus
    Double-Stranded RNA
    Viruses
    Interferons
    Interferon Regulatory Factor-3
    Toll-Like Receptor 3
    Phosphorylation
    Proteins
    Ubiquitin-Protein Ligases
    Genes
    Transcription
    Poly I-C
    Signal transduction
    Melanoma
    Signal Transduction
    Transcription Factors
    Phosphotransferases
    Chemical activation
    Ligands

    Keywords

    • Animals
    • DEAD-box RNA Helicases
    • HEK293 Cells
    • Humans
    • Interferon Regulatory Factor-3
    • Interferon-beta
    • Mice
    • Nuclear Proteins
    • Protein-Serine-Threonine Kinases
    • RNA, Double-Stranded
    • Respirovirus Infections
    • Sendai virus
    • Signal Transduction
    • Toll-Like Receptor 3
    • Transcription Factors
    • Transcription, Genetic
    • Ubiquitin-Protein Ligases

    Cite this

    Ordureau, Alban ; Enesa, Karine ; Nanda, Sambit ; Le Francois, Brice ; Peggie, Mark ; Prescott, Alan ; Albert, Paul R. ; Cohen, Philip. / DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA. In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 34. pp. 24569-24580.
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    abstract = "Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation-associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1)-dependent phosphorylation and activation of Interferon Regulatory Factor 3 (IRF3) and the E3 ubiquitin ligase Pellino1, which are required for interferon {\ss} (IFN{\ss}) gene transcription. Here, we report that Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). The interaction is independent of the E3 ligase activity of Pellino1, but weakened by the phosphorylation of Pellino1. We show that DEAF1 binds to the IFN{\ss} promoter and to IRF3 and IRF7, that it is required for the transcription of the IFN{\ss} gene and IFN{\ss} secretion in MEFs infected with Sendai virus or transfected with poly(I:C). DEAF1 is also needed for TLR3-dependent IFN{\ss} production. Taken together, our results identify DEAF1 as a novel component of the signal transduction network by which dsRNA of viral origin stimulates IFN{\ss} production.",
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    DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA. / Ordureau, Alban; Enesa, Karine; Nanda, Sambit; Le Francois, Brice; Peggie, Mark; Prescott, Alan; Albert, Paul R.; Cohen, Philip.

    In: Journal of Biological Chemistry, Vol. 288, No. 34, 23.08.2013, p. 24569-24580.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - DEAF1 is a Pellino1-interacting protein required for interferon production by Sendai virus and double-stranded RNA

    AU - Ordureau, Alban

    AU - Enesa, Karine

    AU - Nanda, Sambit

    AU - Le Francois, Brice

    AU - Peggie, Mark

    AU - Prescott, Alan

    AU - Albert, Paul R.

    AU - Cohen, Philip

    PY - 2013/8/23

    Y1 - 2013/8/23

    N2 - Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation-associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1)-dependent phosphorylation and activation of Interferon Regulatory Factor 3 (IRF3) and the E3 ubiquitin ligase Pellino1, which are required for interferon ß (IFNß) gene transcription. Here, we report that Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). The interaction is independent of the E3 ligase activity of Pellino1, but weakened by the phosphorylation of Pellino1. We show that DEAF1 binds to the IFNß promoter and to IRF3 and IRF7, that it is required for the transcription of the IFNß gene and IFNß secretion in MEFs infected with Sendai virus or transfected with poly(I:C). DEAF1 is also needed for TLR3-dependent IFNß production. Taken together, our results identify DEAF1 as a novel component of the signal transduction network by which dsRNA of viral origin stimulates IFNß production.

    AB - Double-stranded (ds) RNA of viral origin, a ligand for Melanoma Differentiation-associated gene 5 (MDA5) and Toll-Like Receptor 3 (TLR3), induces the TANK-Binding Kinase 1 (TBK1)-dependent phosphorylation and activation of Interferon Regulatory Factor 3 (IRF3) and the E3 ubiquitin ligase Pellino1, which are required for interferon ß (IFNß) gene transcription. Here, we report that Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). The interaction is independent of the E3 ligase activity of Pellino1, but weakened by the phosphorylation of Pellino1. We show that DEAF1 binds to the IFNß promoter and to IRF3 and IRF7, that it is required for the transcription of the IFNß gene and IFNß secretion in MEFs infected with Sendai virus or transfected with poly(I:C). DEAF1 is also needed for TLR3-dependent IFNß production. Taken together, our results identify DEAF1 as a novel component of the signal transduction network by which dsRNA of viral origin stimulates IFNß production.

    KW - Animals

    KW - DEAD-box RNA Helicases

    KW - HEK293 Cells

    KW - Humans

    KW - Interferon Regulatory Factor-3

    KW - Interferon-beta

    KW - Mice

    KW - Nuclear Proteins

    KW - Protein-Serine-Threonine Kinases

    KW - RNA, Double-Stranded

    KW - Respirovirus Infections

    KW - Sendai virus

    KW - Signal Transduction

    KW - Toll-Like Receptor 3

    KW - Transcription Factors

    KW - Transcription, Genetic

    KW - Ubiquitin-Protein Ligases

    U2 - 10.1074/jbc.M113.479550

    DO - 10.1074/jbc.M113.479550

    M3 - Article

    VL - 288

    SP - 24569

    EP - 24580

    JO - Journal of Biological Chemistry

    JF - Journal of Biological Chemistry

    SN - 0021-9258

    IS - 34

    ER -