Deduced amino acid sequence, gene structure and chromosomal location of a novel human class Mu glutathione S-transferase, GSTM4

S Zhong, N K Spurr, J D Hayes, C R Wolf

    Research output: Contribution to journalArticlepeer-review

    43 Citations (Scopus)

    Abstract

    The Mu-Class glutathione S-transferases (GSTs) are subject to marked inter-individual variation in man, owing to the fact that 40-50% of the population fail to express M1 subunits. Mu-Class GST from two lymphoblastoid cell lines (expressing M1 subunits and the other 'nulled' for M1) have been studied. Both cell lines were found to express a Mu-Class GST that has not been described previously. The cDNA encoding this novel transferase, designated 'GSTM4' has been isolated and the enzyme shown to be comprised of 218 amino acids (including the initiator methionine residue) with an M(r) of approx. 25.5 kDa. Molecular cloning demonstrated that the lymphoblastoid cell line which expressed GSTM1 possessed the b allelic variant (i.e. that with an asparagine residue at position 173). The genes for GSTM4 and GSTM1b have been cloned and found to contain seven introns and eight exons. The coding region of the GSTM4 gene, including the seven introns, encompasses 5.0 kb, whereas the same region of GSTM1b is 5.5 kb; the difference in the size of the two genes is due to the length of intron 7. DNA sequencing allowed a GSTM4-gene-specific oligo-primer to be designed which has been utilized in a PCR-based assay to determine that the GSTM4 gene is located on chromosome 1.

    Original languageEnglish
    Pages (from-to)41-50
    Number of pages10
    JournalBiochemical Journal
    Volume291 ( Pt 1)
    DOIs
    Publication statusPublished - 1 Apr 1993

    Keywords

    • Amino Acid Sequence
    • Cell Line, Transformed
    • Chromosome Mapping
    • Chromosomes, Human, Pair 1
    • Cloning, Molecular
    • DNA/chemistry
    • Exons
    • Glutathione Transferase/chemistry
    • Humans
    • Introns
    • Lymphocytes/enzymology
    • Molecular Sequence Data
    • Polymerase Chain Reaction

    Fingerprint Dive into the research topics of 'Deduced amino acid sequence, gene structure and chromosomal location of a novel human class Mu glutathione S-transferase, GSTM4'. Together they form a unique fingerprint.

    Cite this