TY - JOUR
T1 - Delineation of Paraburkholderia tuberum sensu stricto and description of Paraburkholderia podalyriae sp. nov. nodulating the South African legume Podalyria calyptrata
AU - Mavima, Lazarus
AU - Beukes, Chrizelle W.
AU - Palmer, Marike
AU - De Meyer, Sofie E.
AU - James, Euan K.
AU - Maluk, Marta
AU - Muasya, Muthama A.
AU - Avontuur, Juanita R.
AU - Chan, Wai Yin
AU - Venter, Stephanus N.
AU - Steenkamp, Emma T.
N1 - Funding Information:
The authors would like to acknowledge the National Research Foundation (NRF), the Department of Science and Innovation (DSI) of South Africa and the German Academic Exchange Service (DAAD) for financial support. The authors also extend their gratitude to the Laboratory for Microscopy and Microanalysis Unit of the University of Pretoria for providing electron microscopy infrastructure and guidance. Whole genome sequencing was provided by MicrobesNG ( http://www.microbesng.uk ), which is supported by the BBSRC (grant number BB/L024209/1). The authors would also like to acknowledge Dr B. Lemaire for his isolation of several strains featured in this study.
Funding Information:
The authors would like to acknowledge the National Research Foundation (NRF), the Department of Science and Innovation (DSI) of South Africa and the German Academic Exchange Service (DAAD) for financial support. The authors also extend their gratitude to the Laboratory for Microscopy and Microanalysis Unit of the University of Pretoria for providing electron microscopy infrastructure and guidance. Whole genome sequencing was provided by MicrobesNG (http://www.microbesng.uk), which is supported by the BBSRC (grant number BB/L024209/1). The authors would also like to acknowledge Dr B. Lemaire for his isolation of several strains featured in this study.
Publisher Copyright:
© 2022 Elsevier GmbH
PY - 2022/5
Y1 - 2022/5
N2 - Since the discovery of Paraburkholderia tuberum, an indigenous South African species and one of the first beta-rhizobia described, several other South African rhizobial Paraburkholderia species have been recognized. Here, we investigate the taxonomic status of 31 rhizobial isolates from the root nodules of diverse South African legume hosts in the Core Cape Subregion, which were initially identified as P. tuberum. These isolates originate from the root nodules of genera in the Papilionoideae as well as Vachellia karroo, from the subfamily Caesalpinioideae. Genealogical concordance analysis of five loci allowed delineation of the isolates into two putative species clusters (A and B). Cluster A included P. tuberum STM678T, suggesting that this monophyletic group represents P. tuberum sensu stricto. Cluster B grouped sister to P. tuberum and included isolates from the Paarl Rock Nature Reserve in the Western Cape Province. Average Nucleotide Identity (ANI) analysis further confirmed that isolates of Cluster A shared high genome similarity with P. tuberum STM678T compared to Cluster B and other Paraburkholderia species. The members of Cluster B associated with a single species of Podalyria, P. calyptrata. For this new taxon we accordingly propose the name Paraburkholderia podalyriae sp. nov., with the type strain WC7.3bT (= LMG 31413T; SARCC 750T). Based on our nodA and nifH phylogenies, P. podalyriae sp. nov. and strains of P. tuberum sensu stricto (including one from V. karroo) belong to symbiovar africana, the symbiotic loci of which have a separate evolutionary origin to those of Central and South American Paraburkholderia strains.
AB - Since the discovery of Paraburkholderia tuberum, an indigenous South African species and one of the first beta-rhizobia described, several other South African rhizobial Paraburkholderia species have been recognized. Here, we investigate the taxonomic status of 31 rhizobial isolates from the root nodules of diverse South African legume hosts in the Core Cape Subregion, which were initially identified as P. tuberum. These isolates originate from the root nodules of genera in the Papilionoideae as well as Vachellia karroo, from the subfamily Caesalpinioideae. Genealogical concordance analysis of five loci allowed delineation of the isolates into two putative species clusters (A and B). Cluster A included P. tuberum STM678T, suggesting that this monophyletic group represents P. tuberum sensu stricto. Cluster B grouped sister to P. tuberum and included isolates from the Paarl Rock Nature Reserve in the Western Cape Province. Average Nucleotide Identity (ANI) analysis further confirmed that isolates of Cluster A shared high genome similarity with P. tuberum STM678T compared to Cluster B and other Paraburkholderia species. The members of Cluster B associated with a single species of Podalyria, P. calyptrata. For this new taxon we accordingly propose the name Paraburkholderia podalyriae sp. nov., with the type strain WC7.3bT (= LMG 31413T; SARCC 750T). Based on our nodA and nifH phylogenies, P. podalyriae sp. nov. and strains of P. tuberum sensu stricto (including one from V. karroo) belong to symbiovar africana, the symbiotic loci of which have a separate evolutionary origin to those of Central and South American Paraburkholderia strains.
KW - Beta-rhizobia
KW - Genealogical concordance
KW - Symbiotic loci
KW - Symbiovar
KW - nifH
KW - nodA
UR - http://www.scopus.com/inward/record.url?scp=85126852846&partnerID=8YFLogxK
U2 - 10.1016/j.syapm.2022.126316
DO - 10.1016/j.syapm.2022.126316
M3 - Article
C2 - 35339818
SN - 0723-2020
VL - 45
JO - Systematic and Applied Microbiology
JF - Systematic and Applied Microbiology
IS - 3
M1 - 126316
ER -