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• The cytoplasmic effector Pi04314, expressed as an mRFP-fusion protein with a signal peptide to secrete it from plant cells, does not passively re-enter the cells upon secretion. However, Pi04314-mRFP expressed in P. infestans is translocated from haustoria, which form intimate interactions with plant cells, to accumulate at its sites-of-action in the host nucleus. The well-characterised apoplastic effector EPIC1 was also secreted from haustoria. EPIC1 secretion was inhibited by brefeldin A (BFA), demonstrating that it is delivered by conventional Golgi-mediated secretion. In contrast, Pi04314 secretion was insensitive to BFA treatment, indicating that the cytoplasmic effector follows an alternative route for delivery into plant cells.
• P. infestans haustoria are thus sites for delivery of both apoplastic and cytoplasmic effectors during infection, following distinct secretion pathways.
- effector translocation
- plant disease
- plant immunity
- effector-triggered susceptibility
- apoplastic effector
Supervisor: Birch, P. (Supervisor), Boevink, P. (Supervisor) & Whisson, S. (Supervisor)
Student thesis: Doctoral Thesis › Doctor of Philosophy