Delivery of macromolecules to plant parasitic nematodes using a tobacco rattle virus vector

Tracy A. Valentine, Eva Randall, Krzysztof Wypijewski, Sean Chapman, John Jones, Karl J. Oparka

    Research output: Contribution to journalArticle

    29 Citations (Scopus)

    Abstract

    Plant parasitic nematodes cause significant damage to crops on a worldwide scale. These nematodes are often soil dwelling but rely on plants for food and to sustain them during reproduction. Complex interactions occur between plants and nematodes during the nematode life cycle with plant roots developing specialized feeding structures through which nematodes withdraw nutrients. Here we describe a novel method for delivering macromolecules to feeding nematodes using a virus-based vector [tobacco rattle virus (TRV)]. We show that the parasitic nematode Heterodera schachtii will ingest fluorescent proteins transiently expressed in plant roots infected with a TRV construct carrying the appropriate protein sequence. A prerequisite for this delivery is the presence of replicating virus in root tips prior to the formation of nematode-induced syncytia. We show also that TRV vectors expressing nematode gene sequences can be used to induce RNAi in the feeding nematodes.
    Original languageEnglish
    Pages (from-to)827-834
    Number of pages8
    JournalPlant Biotechnology Journal
    Volume5
    Issue number6
    DOIs
    Publication statusPublished - Nov 2007

    Keywords

    • Animals
    • Arabidopsis
    • Feeding Behavior
    • Gene Expression
    • Giant Cells
    • Peptides
    • Pest Control, Biological
    • Plant Viruses
    • RNA Interference
    • RNA, Double-Stranded
    • Tobacco
    • Tylenchoidea

    Fingerprint Dive into the research topics of 'Delivery of macromolecules to plant parasitic nematodes using a tobacco rattle virus vector'. Together they form a unique fingerprint.

  • Cite this

    Valentine, T. A., Randall, E., Wypijewski, K., Chapman, S., Jones, J., & Oparka, K. J. (2007). Delivery of macromolecules to plant parasitic nematodes using a tobacco rattle virus vector. Plant Biotechnology Journal, 5(6), 827-834. https://doi.org/10.1111/j.1467-7652.2007.00289.x