Demonstration of a tandem pair of complement protein modules in GABAB receptor 1a

Edward Hawrot, Yuanyuan Xiao, Qing Luo Shi, David Norman, Marina Kirkitadze, Paul N. Barlow (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    68 Citations (Scopus)


    We have subcloned and expressed the N-terminal portion of the recently sequenced metabotropic GABA receptor, GABABR1a. This region of the receptor contains a complement protein-like amino acid sequence. The purified 140-residue recombinant protein fragment was soluble and stable. Mass spectrometry indicated formation of four disulfide bonds, as expected if two complement protein modules (CPs, also known as SCRs, Sushi domains) are formed. The circular dichroism spectrum was unusual and characteristic of CPs. Differential scanning calorimetry demonstrated a melting point (64°C), and total enthalpy commensurate with two fully folded domains. We thus conclude that the 1a subtype of the GABAB receptor, but not the 1b subtype, contains a pair of CPs and we present a three-dimensional model of this region. Copyright (C) 1998 Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)103-108
    Number of pages6
    JournalFEBS Letters
    Issue number3
    Publication statusPublished - 7 Aug 1998


    • Drug target
    • Epilepsy
    • GABA(B) receptor
    • SCR
    • Sushi

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology


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