Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy using a targeted imaging probe

Dale J. Waterhouse, James Joseph, Andre A. Neves, Massimiliano di Pietro, Kevin M. Brindle, Rebecca C. Fitzgerald, Sarah E. Bohndiek

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Barrett's esophagus is a condition that predisposes patients to esophageal cancer. Early detection of cancer in these patients can be curative, but is confounded by a lack of contrast in white light endoscopy (WLE). Application of fluorescently-labeled lectins to the esophagus during endoscopy can more accurately delineate dysplasia emerging within Barrett's than WLE1, but strong tissue autofluorescence has limited sensitivity and dynamic range of this approach. To overcome this challenge, we synthesized a near-infrared (NIR) fluorescent lectin and have constructed a clinically translatable endoscope for simultaneous WLE and NIR imaging. An imaging fiber bundle, shielded from patient contact using a disposable catheter, relays collected light into an optical path that splits the WL reflectance and NIR emission onto two cameras for simultaneous video-rate recording. The captured images are co-registered and the honeycomb artifact arising from the fiber bundle is removed using interpolation between image points derived from individual fibers. A minimum detectable concentration of 110 nM was determined using a dilution series of IRDye800CW-lectin in black well plates. We have demonstrated the ability to use our endoscope to distinguish between different tissue types in ex vivo mouse stomachs. Future work using human ex vivo tissue specimens will determine safe illumination limits and sensitivity for dysplasia and adenocarcinoma in Barrett's esophagus, prior to commencing clinical trials.

Original languageEnglish
Title of host publicationAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV
EditorsAnita Mahadevan-Jansen, Tuan Vo-Dinh, Warren S. Grundfest
PublisherSociety of Photo-optical Instrumentation Engineers
Number of pages9
ISBN (Electronic)9781628419320
DOIs
Publication statusPublished - 7 Mar 2016
EventAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV - San Francisco, United States
Duration: 14 Feb 201616 Feb 2016

Publication series

NameProceedings of SPIE
Volume9698
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV
CountryUnited States
CitySan Francisco
Period14/02/1616/02/16

Keywords

  • Barrett's esophagus
  • clinical translation
  • dysplasia
  • Flexible endoscope
  • fluorescence
  • near infrared

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  • Cite this

    Waterhouse, D. J., Joseph, J., Neves, A. A., di Pietro, M., Brindle, K. M., Fitzgerald, R. C., & Bohndiek, S. E. (2016). Design and validation of a near-infrared fluorescence endoscope for detection of early esophageal malignancy using a targeted imaging probe. In A. Mahadevan-Jansen, T. Vo-Dinh, & W. S. Grundfest (Eds.), Advanced Biomedical and Clinical Diagnostic and Surgical Guidance Systems XIV [969803] (Proceedings of SPIE; Vol. 9698). Society of Photo-optical Instrumentation Engineers. https://doi.org/10.1117/12.2209582