Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues

M.P. Khoury, V. Marcel, K. Fernandes, A. Diot, D.P. Lane, J.-C. Bourdon

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    10 Citations (Scopus)

    Abstract

    The TP53 gene expresses at least nine different mRNA variants (p53 isoform mRNAs), including the one encoding the canonical p53 tumor suppressor protein. We have developed scientific tools to specifically detect and quantify p53 isoform expression at mRNA level by nested RT-PCR (reverse transcription- polymerase chain reaction) and quantitative real-time RT-PCR (RT-qPCR using the TaqMan chemistry). Here, we describe these two methods, while highlighting essential points with regard to the analysis of p53 isoform mRNA expression.
    Original languageEnglish
    Title of host publicationp53 protocols
    EditorsSumitra Deb, Swati Palit Deb
    Place of PublicationTotowa, N.J.
    PublisherHumana Press
    Pages1-14
    Number of pages14
    ISBN (Print)9781627032353
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume962

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  • Cite this

    Khoury, M. P., Marcel, V., Fernandes, K., Diot, A., Lane, D. P., & Bourdon, J-C. (2013). Detecting and quantifying p53 isoforms at mRNA level in cell lines and tissues. In S. Deb, & S. P. Deb (Eds.), p53 protocols (pp. 1-14). (Methods in Molecular Biology; Vol. 962). Humana Press. https://doi.org/10.1007/978-1-62703-236-0_1