Detecting and Segmenting Nanodiscs in Immuno-Electron Micrographs

Tianjun Huang, Christian Hacker, John Lucocq, Stephen McKenna

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Abstract

Nanodiscs are soluble nanoscale phospholipid bilayers with applications in drug delivery and the study of membrane proteins, for example. They can be imaged using electron microscopy, along with immunogold markers indicating locations of proteins of interest. We describe and evaluate methods for automatically detecting and segmenting nanodiscs in electron micrographs. The detection method modifies aspects of the Fast Radial Symmetry Transform to detect nanodiscs that exhibit approximate radial symmetry against noisy but predominantly lighter background. Detected nanodiscs are then segmented using radial active contours. Experiments on micrographs both with and without immunogold markers indicate promising detection and segmentation performance, and that information on nanodisc quantities, locations, size distributions, and co-location with proteins of interest could be extracted automatically.
Original languageEnglish
Title of host publicationMedical Image Understanding and Analysis
Subtitle of host publicationMIUA
EditorsConstantino Reyes-Aldasoro, Greg Slabaugh
Place of PublicationUnited Kingdom
PublisherBritish Machine Vision Association and Society for Pattern Recognition
Pages53-58
Number of pages6
ISBN (Print)1901725510
Publication statusPublished - Jul 2014
Event18th Annual Conference in Medical Image Understanding and Analysis - Moore Complex, Royal Holloway, London, United Kingdom
Duration: 9 Jul 201411 Jul 2014
http://www.city.ac.uk/medical-image-understanding-and-analysis-2014

Conference

Conference18th Annual Conference in Medical Image Understanding and Analysis
Abbreviated titleMIUA 2014
CountryUnited Kingdom
CityLondon
Period9/07/1411/07/14
Internet address

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