@inbook{31b35d2d1d73471b93191cfe79777b73,
title = "Detecting p53 isoforms at protein level",
abstract = "The human p53 protein isoforms are expressed in several cell lines and modulate p53 tumor suppressor activity, mainly through modulation of gene expression (1-4). Thus, identifying the pattern of p53 isoforms expression in cell lines is a key step for future studies of the p53 network (5). At the moment, the detection of p53 protein isoforms is based on the use of a panel of antibodies allowing their identification by comparing their molecular weights and their detection pattern by different antibodies (6). Here, classical protocols supplemented with technical know-how are described to detect p53 protein isoforms at protein level by Western blotting and immunoprecipitation. Furthermore, a simple method to study the impact of p53 protein isoforms on p53 transcriptional activity through luciferase reporter gene assays is provided.",
author = "V. Marcel and M.P. Khoury and K. Fernandes and A. Diot and D.P. Lane and J.-C. Bourdon",
year = "2013",
doi = "10.1007/978-1-62703-236-0_2",
language = "English",
isbn = "9781627032353 ",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "15--29",
editor = "Sumitra Deb and Deb, {Swati Palit}",
booktitle = "p53 protocols",
address = "United States",
}