Detecting p53 isoforms at protein level

V. Marcel, M.P. Khoury, K. Fernandes, A. Diot, D.P. Lane, J.-C. Bourdon

    Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

    13 Citations (Scopus)

    Abstract

    The human p53 protein isoforms are expressed in several cell lines and modulate p53 tumor suppressor activity, mainly through modulation of gene expression (1-4). Thus, identifying the pattern of p53 isoforms expression in cell lines is a key step for future studies of the p53 network (5). At the moment, the detection of p53 protein isoforms is based on the use of a panel of antibodies allowing their identification by comparing their molecular weights and their detection pattern by different antibodies (6). Here, classical protocols supplemented with technical know-how are described to detect p53 protein isoforms at protein level by Western blotting and immunoprecipitation. Furthermore, a simple method to study the impact of p53 protein isoforms on p53 transcriptional activity through luciferase reporter gene assays is provided.
    Original languageEnglish
    Title of host publicationp53 protocols
    EditorsSumitra Deb, Swati Palit Deb
    Place of PublicationTotowa, N.J.
    PublisherHumana Press
    Pages15-29
    Number of pages15
    ISBN (Print)9781627032353
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    PublisherHumana Press
    Volume962

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  • Cite this

    Marcel, V., Khoury, M. P., Fernandes, K., Diot, A., Lane, D. P., & Bourdon, J-C. (2013). Detecting p53 isoforms at protein level. In S. Deb, & S. P. Deb (Eds.), p53 protocols (pp. 15-29). (Methods in Molecular Biology; Vol. 962). Humana Press. https://doi.org/10.1007/978-1-62703-236-0_2