Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Helen Kulesza; Gordon G. Simpson; Robbie Waugh; Jean D. Beggs; John W.S. Brown

doi:10.1016/0014-5793(93)81315-Q

Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Helen Kulesza, Gordon G. Simpson, Robbie Waugh, Jean D. Beggs, John W.S. Brown (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

Original language	English
Pages (from-to)	4-6
Number of pages	3
Journal	FEBS Letters
Volume	318
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(93)81315-Q
Publication status	Published - 22 Feb 1993

Keywords

Affinity-purified antibodies
Pre-mRNA splicing
PRP8
snRNP
Spliceosomal protein
Tobacco

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8",

abstract = "We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.",

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AU - Kulesza, Helen

AU - Simpson, Gordon G.

AU - Waugh, Robbie

AU - Beggs, Jean D.

AU - Brown, John W.S.

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AB - We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

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Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Abstract

Keywords

ASJC Scopus subject areas

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