Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Helen Kulesza, Gordon G. Simpson, Robbie Waugh, Jean D. Beggs, John W.S. Brown (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

Original languageEnglish
Pages (from-to)4-6
Number of pages3
JournalFEBS Letters
Volume318
Issue number1
DOIs
Publication statusPublished - 22 Feb 1993

Keywords

  • Affinity-purified antibodies
  • Pre-mRNA splicing
  • PRP8
  • snRNP
  • Spliceosomal protein
  • Tobacco

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