Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Helen Kulesza; Gordon G. Simpson; Robbie Waugh; Jean D. Beggs; John W.S. Brown

doi:10.1016/0014-5793(93)81315-Q

Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Helen Kulesza
, Gordon G. Simpson
, Robbie Waugh
, Jean D. Beggs
, John W.S. Brown (Lead / Corresponding author)

Plant Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

Original language	English
Pages (from-to)	4-6
Number of pages	3
Journal	FEBS Letters
Volume	318
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(93)81315-Q
Publication status	Published - 22 Feb 1993

Keywords

Affinity-purified antibodies
Pre-mRNA splicing
PRP8
snRNP
Spliceosomal protein
Tobacco

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(93)81315-Q

Cite this

@article{8324192375114119a5dcfef31218718e,

title = "Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8",

abstract = "We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.",

keywords = "Affinity-purified antibodies, Pre-mRNA splicing, PRP8, snRNP, Spliceosomal protein, Tobacco",

author = "Helen Kulesza and Simpson, \{Gordon G.\} and Robbie Waugh and Beggs, \{Jean D.\} and Brown, \{John W.S.\}",

year = "1993",

month = feb,

day = "22",

doi = "10.1016/0014-5793(93)81315-Q",

language = "English",

volume = "318",

pages = "4--6",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley",

number = "1",

}

TY - JOUR

T1 - Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

AU - Kulesza, Helen

AU - Simpson, Gordon G.

AU - Waugh, Robbie

AU - Beggs, Jean D.

AU - Brown, John W.S.

PY - 1993/2/22

Y1 - 1993/2/22

N2 - We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

AB - We have investigated whether a splieeosomal protein analogous to the yeast protein, PRP8, was present in higher plants. A protein with a molecular weight > 200 kDa was detected in Western blots of tobacco (Nicotiana tabacum L.) nuclear extracts with affinity-purified antibodies, raised against four different β-galactosidase-PRP8 fusion proteins. The > 200 kDa protein was also immunoprecipitated by antibodies against the snRNA-specific trimethylguanosine cap structure and was, therefore, snRNP-associated. The presence of this protein in plants, in addition to yeast, Drosophila and humans, and the conservation of large size and epitopes highlights the importance of PRP8 in pre-mRNA splicing.

KW - Affinity-purified antibodies

KW - Pre-mRNA splicing

KW - PRP8

KW - snRNP

KW - Spliceosomal protein

KW - Tobacco

UR - https://www.scopus.com/pages/publications/0027458698

U2 - 10.1016/0014-5793(93)81315-Q

DO - 10.1016/0014-5793(93)81315-Q

M3 - Article

C2 - 8436222

AN - SCOPUS:0027458698

SN - 0014-5793

VL - 318

SP - 4

EP - 6

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Detection of a plant protein analogous to the yeast spliceosomal protein, PRP8

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this