Detection of Epstein-Barr virus in Reed-Sternberg cells of Hodgkin's disease arising in children

Philip J. Coates, A. Jane d'Ardenne, Gerard Slavin, Judith E. Kingston, James S. Malpas

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    Abstract

    Nonisotopic in situ hybridization has been used to investigate the role of Epstein-Barr virus (EBV) in the aetiology of pediatric Hodgkin's disease. Sections from 24 cases arising in children under the age of 15 years were hybridised with digoxigenin-labelled probes for both EBV and cytomegalovirus, and reactive sites were identified by a sensitive three-layer immunoperoxidase technique. EBV was identified in Reed-Sternberg and mononuclear Hodgkin's cells in five samples (21%). No samples were positive when the cytomegalovirus probe was employed. The specific identification of EBV in the malignant cells of Hodgkin's disease arising in children lends further support for a role of EBV in the aetiology of this disorder.
    Original languageEnglish
    Pages (from-to)19-23
    Number of pages5
    JournalMedical and Pediatric Oncology
    Volume21
    Issue number1
    DOIs
    Publication statusPublished - 1993

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