Detection of Epstein-Barr virus in Reed-Sternberg cells of Hodgkin's disease arising in children

TY - JOUR

T1 - Detection of Epstein-Barr virus in Reed-Sternberg cells of Hodgkin's disease arising in children

AU - Coates, Philip J.

AU - d'Ardenne, A. Jane

AU - Slavin, Gerard

AU - Kingston, Judith E.

AU - Malpas, James S.

PY - 1993

Y1 - 1993

N2 - Nonisotopic in situ hybridization has been used to investigate the role of Epstein-Barr virus (EBV) in the aetiology of pediatric Hodgkin's disease. Sections from 24 cases arising in children under the age of 15 years were hybridised with digoxigenin-labelled probes for both EBV and cytomegalovirus, and reactive sites were identified by a sensitive three-layer immunoperoxidase technique. EBV was identified in Reed-Sternberg and mononuclear Hodgkin's cells in five samples (21%). No samples were positive when the cytomegalovirus probe was employed. The specific identification of EBV in the malignant cells of Hodgkin's disease arising in children lends further support for a role of EBV in the aetiology of this disorder.

AB - Nonisotopic in situ hybridization has been used to investigate the role of Epstein-Barr virus (EBV) in the aetiology of pediatric Hodgkin's disease. Sections from 24 cases arising in children under the age of 15 years were hybridised with digoxigenin-labelled probes for both EBV and cytomegalovirus, and reactive sites were identified by a sensitive three-layer immunoperoxidase technique. EBV was identified in Reed-Sternberg and mononuclear Hodgkin's cells in five samples (21%). No samples were positive when the cytomegalovirus probe was employed. The specific identification of EBV in the malignant cells of Hodgkin's disease arising in children lends further support for a role of EBV in the aetiology of this disorder.

U2 - 10.1002/mpo.2950210105

DO - 10.1002/mpo.2950210105

M3 - Article

C2 - 8381200

SN - 0098-1532

VL - 21

SP - 19

EP - 23

JO - Medical and Pediatric Oncology

JF - Medical and Pediatric Oncology

IS - 1

ER -