Detection of genetic diversity and selective gene introgression in coffee using RAPD markers

C. Orozco-Castillo, K. J. Chalmers, R. Waugh, W. Powell

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)


RAPD (randomly amplified polymorphic DNA) markers generated by arbitary decamers have been successfully employed to detect genetic polymorphisms between coffee species and between Coffea arabica genotypes. The RAPD profiles were used to construct dendrograms and these were consistent with the known history and evolution of Coffea arabica. Material originating from Ethiopia and the arabica sub-groups — C. arabica var. typica and C. arabica var. bourbon — were clearly distinguished. RAPD analysis therefore reflects morphological differences between the sub-groups and the geographical origin of the coffee material. Species-specific amplification products were also identified, but, more importantly, amplification products specific to C. canephora were identified in two C. arabica genotypes, Rume Sudan and Catimor 5175. This diagnostic product is therefore indicative of interspecific gene flow in coffee and has biological implications for selective introgressive hybridisation in coffee. Our study demonstrates the power of the polymerase chain reaction technology for the generation of genetic markers for long-lived perennial tree and bush crops.

Original languageEnglish
Pages (from-to)934-940
Number of pages7
JournalTheoretical and Applied Genetics: International Journal of Plant Breeding Research
Issue number8
Publication statusPublished - 1 Jan 1994


  • Coffee
  • Diversity
  • Gene introgression
  • RAPDs

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics


Dive into the research topics of 'Detection of genetic diversity and selective gene introgression in coffee using RAPD markers'. Together they form a unique fingerprint.

Cite this