Detection of high risk human papillomavirus in routine cervical smears

strategy for screening

C. S. Herrington, M. de Angelis, M. F. Evans, G. Troncone, J. O. McGee

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Aim: To develop a methodology for direct detection of high risk human papillomavirus (HPV) infection in routine cervical smears by non-isotopic in situ hybridisation (NISH) which can be compared with cytopathological assessment of the same cells.

    Methods: The methodology was established using cultured cells and routine cervical smears hybridised with digoxigenin labelled probes for HPV, 16, 18, 31, and 33. The technique was applied to the analysis of 53 patients from a sexually transmitted disease clinic.

    Results: The optimal sensitivity achieved for single HPV detection in cultured cells was 1-2 copies of HPV 16 per cell and that for detection of a cocktail of HPV types in routine cervical smears was 2.5-12 copies per cell. Of parallel smears taken from patients with a normal Papinacolau-stained smear 33.3% (24) contained a HPV 16, 18, 31, and 33 signal indicating an occult HPV infection. The prevalence of these HPV types was similar in women in whom a cytopathological diagnosis of wart virus infection was made (64.7%, 17) and in patients with mild dyskaryosis (75%, 12).

    Conclusions: The methodology evolved localises HPV sequences directly to epithelial cell nuclei, which can be morphologically assessed by haematoxylin counterstaining. Sample contamination with exogenous viral sequences can be distinguished from true infection. In this study, a HPV signal was not found in morphologically normal epithelial cells. The methods described will permit the detection of HPV sequences in routinely collected cervical smears and the evaluation of the natural history and potential clinical relevance of HPV infection without changes in clinical practice.

    Original languageEnglish
    Pages (from-to)385-390
    Number of pages6
    JournalJournal of Clinical Pathology
    Volume45
    Issue number5
    DOIs
    Publication statusPublished - 1992

    Cite this

    Herrington, C. S., de Angelis, M., Evans, M. F., Troncone, G., & McGee, J. O. (1992). Detection of high risk human papillomavirus in routine cervical smears: strategy for screening. Journal of Clinical Pathology, 45(5), 385-390. https://doi.org/10.1136/jcp.45.5.385
    Herrington, C. S. ; de Angelis, M. ; Evans, M. F. ; Troncone, G. ; McGee, J. O. . / Detection of high risk human papillomavirus in routine cervical smears : strategy for screening. In: Journal of Clinical Pathology. 1992 ; Vol. 45, No. 5. pp. 385-390.
    @article{26193885980545729db89eb2bf8c99f1,
    title = "Detection of high risk human papillomavirus in routine cervical smears: strategy for screening",
    abstract = "Aim: To develop a methodology for direct detection of high risk human papillomavirus (HPV) infection in routine cervical smears by non-isotopic in situ hybridisation (NISH) which can be compared with cytopathological assessment of the same cells.Methods: The methodology was established using cultured cells and routine cervical smears hybridised with digoxigenin labelled probes for HPV, 16, 18, 31, and 33. The technique was applied to the analysis of 53 patients from a sexually transmitted disease clinic.Results: The optimal sensitivity achieved for single HPV detection in cultured cells was 1-2 copies of HPV 16 per cell and that for detection of a cocktail of HPV types in routine cervical smears was 2.5-12 copies per cell. Of parallel smears taken from patients with a normal Papinacolau-stained smear 33.3{\%} (24) contained a HPV 16, 18, 31, and 33 signal indicating an occult HPV infection. The prevalence of these HPV types was similar in women in whom a cytopathological diagnosis of wart virus infection was made (64.7{\%}, 17) and in patients with mild dyskaryosis (75{\%}, 12).Conclusions: The methodology evolved localises HPV sequences directly to epithelial cell nuclei, which can be morphologically assessed by haematoxylin counterstaining. Sample contamination with exogenous viral sequences can be distinguished from true infection. In this study, a HPV signal was not found in morphologically normal epithelial cells. The methods described will permit the detection of HPV sequences in routinely collected cervical smears and the evaluation of the natural history and potential clinical relevance of HPV infection without changes in clinical practice.",
    author = "Herrington, {C. S.} and {de Angelis}, M. and Evans, {M. F.} and G. Troncone and McGee, {J. O.}",
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    Herrington, CS, de Angelis, M, Evans, MF, Troncone, G & McGee, JO 1992, 'Detection of high risk human papillomavirus in routine cervical smears: strategy for screening', Journal of Clinical Pathology, vol. 45, no. 5, pp. 385-390. https://doi.org/10.1136/jcp.45.5.385

    Detection of high risk human papillomavirus in routine cervical smears : strategy for screening. / Herrington, C. S.; de Angelis, M. ; Evans, M. F. ; Troncone, G. ; McGee, J. O. .

    In: Journal of Clinical Pathology, Vol. 45, No. 5, 1992, p. 385-390.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Detection of high risk human papillomavirus in routine cervical smears

    T2 - strategy for screening

    AU - Herrington, C. S.

    AU - de Angelis, M.

    AU - Evans, M. F.

    AU - Troncone, G.

    AU - McGee, J. O.

    PY - 1992

    Y1 - 1992

    N2 - Aim: To develop a methodology for direct detection of high risk human papillomavirus (HPV) infection in routine cervical smears by non-isotopic in situ hybridisation (NISH) which can be compared with cytopathological assessment of the same cells.Methods: The methodology was established using cultured cells and routine cervical smears hybridised with digoxigenin labelled probes for HPV, 16, 18, 31, and 33. The technique was applied to the analysis of 53 patients from a sexually transmitted disease clinic.Results: The optimal sensitivity achieved for single HPV detection in cultured cells was 1-2 copies of HPV 16 per cell and that for detection of a cocktail of HPV types in routine cervical smears was 2.5-12 copies per cell. Of parallel smears taken from patients with a normal Papinacolau-stained smear 33.3% (24) contained a HPV 16, 18, 31, and 33 signal indicating an occult HPV infection. The prevalence of these HPV types was similar in women in whom a cytopathological diagnosis of wart virus infection was made (64.7%, 17) and in patients with mild dyskaryosis (75%, 12).Conclusions: The methodology evolved localises HPV sequences directly to epithelial cell nuclei, which can be morphologically assessed by haematoxylin counterstaining. Sample contamination with exogenous viral sequences can be distinguished from true infection. In this study, a HPV signal was not found in morphologically normal epithelial cells. The methods described will permit the detection of HPV sequences in routinely collected cervical smears and the evaluation of the natural history and potential clinical relevance of HPV infection without changes in clinical practice.

    AB - Aim: To develop a methodology for direct detection of high risk human papillomavirus (HPV) infection in routine cervical smears by non-isotopic in situ hybridisation (NISH) which can be compared with cytopathological assessment of the same cells.Methods: The methodology was established using cultured cells and routine cervical smears hybridised with digoxigenin labelled probes for HPV, 16, 18, 31, and 33. The technique was applied to the analysis of 53 patients from a sexually transmitted disease clinic.Results: The optimal sensitivity achieved for single HPV detection in cultured cells was 1-2 copies of HPV 16 per cell and that for detection of a cocktail of HPV types in routine cervical smears was 2.5-12 copies per cell. Of parallel smears taken from patients with a normal Papinacolau-stained smear 33.3% (24) contained a HPV 16, 18, 31, and 33 signal indicating an occult HPV infection. The prevalence of these HPV types was similar in women in whom a cytopathological diagnosis of wart virus infection was made (64.7%, 17) and in patients with mild dyskaryosis (75%, 12).Conclusions: The methodology evolved localises HPV sequences directly to epithelial cell nuclei, which can be morphologically assessed by haematoxylin counterstaining. Sample contamination with exogenous viral sequences can be distinguished from true infection. In this study, a HPV signal was not found in morphologically normal epithelial cells. The methods described will permit the detection of HPV sequences in routinely collected cervical smears and the evaluation of the natural history and potential clinical relevance of HPV infection without changes in clinical practice.

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    DO - 10.1136/jcp.45.5.385

    M3 - Article

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    SP - 385

    EP - 390

    JO - Journal of Clinical Pathology

    JF - Journal of Clinical Pathology

    SN - 0021-9746

    IS - 5

    ER -