Detection of Post-translationally Modified p53 by Western Blotting

Anna Estevan Barber, David W. Meek (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review


The p53 tumor suppressor has a central role in many key cellular processes including the DNA damage response, aging, stem cell differentiation, and fertility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation, acetylation, methylation, and ubiquitylation. Here, we describe western blotting-based methodology for the detection and relative quantification of individual phosphorylation events in p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies.

Original languageEnglish
Title of host publicationCell Cycle Checkpoints
Subtitle of host publicationMethods and Protocols
EditorsJames J. Manfredi
Place of PublicationNew York
PublisherHumana Press
Number of pages12
ISBN (Electronic)9781071612170
ISBN (Print)9781071612163 (hbk), 9781071612194 (pbk)
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • DNA-damage response
  • Phospho-specific antibodies
  • Phosphorylation
  • Post-translational modifications
  • Western blot
  • p53

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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