@inbook{5a8e230bd3cd468dadbe7f7e308c7f74,
title = "Detection of Post-translationally Modified p53 by Western Blotting",
abstract = "The p53 tumor suppressor has a central role in many key cellular processes including the DNA damage response, aging, stem cell differentiation, and fertility. p53 undergoes extensive regulatory post-translational modification through events such as phosphorylation, acetylation, methylation, and ubiquitylation. Here, we describe western blotting-based methodology for the detection and relative quantification of individual phosphorylation events in p53. While we focus on well-established N-terminal modifications for the purpose of illustration, this approach can be used to investigate other post-translational modifications of the protein, drawing upon a broad range of commercially available modification-specific antibodies.",
keywords = "DNA-damage response, Phospho-specific antibodies, Phosphorylation, Post-translational modifications, Western blot, p53",
author = "Barber, {Anna Estevan} and Meek, {David W.}",
note = "Funding Information: This work was supported by a studentship from Breast Cancer Now (UK), grant number: 2013NovPhD134. Publisher Copyright: {\textcopyright} 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
doi = "10.1007/978-1-0716-1217-0_2",
language = "English",
isbn = "9781071612163 (hbk)",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "7--18",
editor = "Manfredi, {James J.}",
booktitle = "Cell Cycle Checkpoints",
edition = "1",
}