TY - JOUR
T1 - Detection of protein complex interactions via a blue native-page retardation assay
AU - Swamy, Mahima
AU - Molnar, Eszter
AU - Bock, Thomas
AU - Bausch-Fluck, Damaris
AU - Wollscheid, Bernd
AU - Schamel, Wolfgang W.A.
PY - 2009/9/15
Y1 - 2009/9/15
N2 - We describe the Blue Native (BN)-PAGE retardation assay for the detection of interactions of biomolecules with protein complexes. Potential interactors of proteins are included in the BN gel matrix, resulting in retardation of proteins that interact with the added molecule. After validation using the T-cell antigen receptor, we applied the assay for a general identification of dextran interactors in combination with mass spectroscopy. The proteomic screen revealed triosephosphate isomerase oligomer as a dextran-binding, high MR complex.
AB - We describe the Blue Native (BN)-PAGE retardation assay for the detection of interactions of biomolecules with protein complexes. Potential interactors of proteins are included in the BN gel matrix, resulting in retardation of proteins that interact with the added molecule. After validation using the T-cell antigen receptor, we applied the assay for a general identification of dextran interactors in combination with mass spectroscopy. The proteomic screen revealed triosephosphate isomerase oligomer as a dextran-binding, high MR complex.
UR - http://www.scopus.com/inward/record.url?scp=67650474854&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2009.05.039
DO - 10.1016/j.ab.2009.05.039
M3 - Article
C2 - 19482002
AN - SCOPUS:67650474854
SN - 0003-2697
VL - 392
SP - 177
EP - 179
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -