Detection of protein complex interactions via a blue native-page retardation assay

Mahima Swamy, Eszter Molnar, Thomas Bock, Damaris Bausch-Fluck, Bernd Wollscheid, Wolfgang W.A. Schamel (Lead / Corresponding author)

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We describe the Blue Native (BN)-PAGE retardation assay for the detection of interactions of biomolecules with protein complexes. Potential interactors of proteins are included in the BN gel matrix, resulting in retardation of proteins that interact with the added molecule. After validation using the T-cell antigen receptor, we applied the assay for a general identification of dextran interactors in combination with mass spectroscopy. The proteomic screen revealed triosephosphate isomerase oligomer as a dextran-binding, high MR complex.

Original languageEnglish
Pages (from-to)177-179
Number of pages3
JournalAnalytical Biochemistry
Volume392
Issue number2
DOIs
Publication statusPublished - 15 Sep 2009

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    Swamy, M., Molnar, E., Bock, T., Bausch-Fluck, D., Wollscheid, B., & Schamel, W. W. A. (2009). Detection of protein complex interactions via a blue native-page retardation assay. Analytical Biochemistry, 392(2), 177-179. https://doi.org/10.1016/j.ab.2009.05.039