Determinants of Myosin II Cortical Localization during Cytokinesis

Ryota Uehara, Gohta Goshima, Issei Mabuchi, Ronald D. Vale, James A. Spudich, Eric R. Griffis (Lead / Corresponding author)

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    59 Citations (Scopus)

    Abstract

    Myosin II is an essential component of the contractile ring that divides the cell during cytokinesis. Previous work showed that regulatory light chain (RLC) phosphorylation is required for localization of myosin at the cellular equator [1, 2]. However, the molecular mechanisms that concentrate myosin at the site of furrow formation remain unclear. By analyzing the spatiotemporal dynamics of mutant myosin subunits in Drosophila S2 cells, we show that myosin accumulates at the equator through stabilization of interactions between the cortex and myosin filaments and that the motor domain is dispensable for localization. Filament stabilization is tightly controlled by RLC phosphorylation. However, we show that regulatory mechanisms other than RLC phosphorylation contribute to myosin accumulation at three different stages: (1) turnover of thick filaments throughout the cell cycle, (2) myosin heavy chain-based control of myosin assembly at the metaphase-anaphase transition, and (3) redistribution and/or activation of myosin binding sites at the equator during anaphase. Surprisingly, the third event can occur to a degree in a Rho-independent fashion, gathering preassembled filaments to the equatorial zone via cortical flow. We conclude that multiple regulatory pathways cooperate to control myosin localization during mitosis and cytokinesis to ensure that this essential biological process is as robust as possible.

    Original languageEnglish
    Pages (from-to)1080-1085
    Number of pages6
    JournalCurrent Biology
    Volume20
    Issue number12
    DOIs
    Publication statusPublished - 22 Jun 2010

    Keywords

    • DROSOPHILA S2 CELLS
    • NONMUSCLE MYOSIN
    • CHAIN PHOSPHORYLATION
    • HEAVY-CHAIN
    • LIGHT-CHAIN
    • FILAMENT FORMATION
    • RECRUITMENT
    • PROTEIN
    • KINASE
    • FURROW

    Cite this

    Uehara, R., Goshima, G., Mabuchi, I., Vale, R. D., Spudich, J. A., & Griffis, E. R. (2010). Determinants of Myosin II Cortical Localization during Cytokinesis. Current Biology, 20(12), 1080-1085. https://doi.org/10.1016/j.cub.2010.04.058