TY - JOUR
T1 - Determination of metals and metal fluxes in algae and fungi
AU - White, C.
AU - Gadd, G. M.
N1 - Funding Information:
GMG gratefully acknowledges receipt of a Scottish Office Education Department/Royal Society of Edinburgh Research Fellowship and financial support from the Natural Environment Research Council, the Science and Engineering Research Council, the Wellcome Burroughs Foundation and the Carnegie Trust for the Universities of Scotland for the work described.
Copyright © 1996 Published by Elsevier B.V.
PY - 1995/12/22
Y1 - 1995/12/22
N2 - Methods available for the determination of metals and metal fluxes in algae and fungi in routine use in our laboratory include atomic absorption spectroscopy (AAS), the use of radioisotope tracers, polarography and ion specific electrodes. The choice of method depends on the particular problem under investigation and is governed by sensitivity, specificity and accuracy for the metal or metal species in question, the concentration range, requirements of sampling and sample preparation and whether there is a need to distinguish cellular elemental location. The application of these analytical techniques to determine capacities and kinetics of metal and radionuclide uptake, intracellular metal distribution, K+ effuux and the biosorption of organotin compounds in cyanobacteria, microalgae and fungi, including yeasts, is described in this paper, with due reference to the experimental contexts most appropriate for a given technique.
AB - Methods available for the determination of metals and metal fluxes in algae and fungi in routine use in our laboratory include atomic absorption spectroscopy (AAS), the use of radioisotope tracers, polarography and ion specific electrodes. The choice of method depends on the particular problem under investigation and is governed by sensitivity, specificity and accuracy for the metal or metal species in question, the concentration range, requirements of sampling and sample preparation and whether there is a need to distinguish cellular elemental location. The application of these analytical techniques to determine capacities and kinetics of metal and radionuclide uptake, intracellular metal distribution, K+ effuux and the biosorption of organotin compounds in cyanobacteria, microalgae and fungi, including yeasts, is described in this paper, with due reference to the experimental contexts most appropriate for a given technique.
KW - Atomic absorption spectroscopy
KW - Ion specific electrodes
KW - Polarography
KW - Radioisotope tracers
UR - http://www.scopus.com/inward/record.url?scp=0029565973&partnerID=8YFLogxK
U2 - 10.1016/0048-9697(95)04836-7
DO - 10.1016/0048-9697(95)04836-7
M3 - Article
AN - SCOPUS:0029565973
SN - 0048-9697
VL - 176
SP - 107
EP - 115
JO - Science of the Total Environment
JF - Science of the Total Environment
IS - 1-3
ER -