Determination of metals and metal fluxes in algae and fungi

TY - JOUR

T1 - Determination of metals and metal fluxes in algae and fungi

AU - White, C.

AU - Gadd, G. M.

N1 - Funding Information: GMG gratefully acknowledges receipt of a Scottish Office Education Department/Royal Society of Edinburgh Research Fellowship and financial support from the Natural Environment Research Council, the Science and Engineering Research Council, the Wellcome Burroughs Foundation and the Carnegie Trust for the Universities of Scotland for the work described. Copyright © 1996 Published by Elsevier B.V.

PY - 1995/12/22

Y1 - 1995/12/22

N2 - Methods available for the determination of metals and metal fluxes in algae and fungi in routine use in our laboratory include atomic absorption spectroscopy (AAS), the use of radioisotope tracers, polarography and ion specific electrodes. The choice of method depends on the particular problem under investigation and is governed by sensitivity, specificity and accuracy for the metal or metal species in question, the concentration range, requirements of sampling and sample preparation and whether there is a need to distinguish cellular elemental location. The application of these analytical techniques to determine capacities and kinetics of metal and radionuclide uptake, intracellular metal distribution, K+ effuux and the biosorption of organotin compounds in cyanobacteria, microalgae and fungi, including yeasts, is described in this paper, with due reference to the experimental contexts most appropriate for a given technique.

AB - Methods available for the determination of metals and metal fluxes in algae and fungi in routine use in our laboratory include atomic absorption spectroscopy (AAS), the use of radioisotope tracers, polarography and ion specific electrodes. The choice of method depends on the particular problem under investigation and is governed by sensitivity, specificity and accuracy for the metal or metal species in question, the concentration range, requirements of sampling and sample preparation and whether there is a need to distinguish cellular elemental location. The application of these analytical techniques to determine capacities and kinetics of metal and radionuclide uptake, intracellular metal distribution, K+ effuux and the biosorption of organotin compounds in cyanobacteria, microalgae and fungi, including yeasts, is described in this paper, with due reference to the experimental contexts most appropriate for a given technique.

KW - Atomic absorption spectroscopy

KW - Ion specific electrodes

KW - Polarography

KW - Radioisotope tracers

UR - https://www.scopus.com/pages/publications/0029565973

U2 - 10.1016/0048-9697(95)04836-7

DO - 10.1016/0048-9697(95)04836-7

M3 - Article

AN - SCOPUS:0029565973

SN - 0048-9697

VL - 176

SP - 107

EP - 115

JO - Science of the Total Environment

JF - Science of the Total Environment

IS - 1-3

ER -