Determining an in vitro dose-response relationship of photodynamic therapy with first and second-generation photosensitisers for high grade tumours

Kirit Singh, Daniel Baptista-Hon, Molly Hewitt, Omar Kouli, Kismet Hossain-Ibrahim, Tim Hales

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photodynamic Therapy (PDT) is used with photosensitizing agents to enhance the extent of tumour destruction intra-operatively, thereby enhancing survival. However, high-grade tumour types demonstrate significant heterogeneity and their susceptibility to PDT may vary. This study aimed to determine the response of different invasive tumour types to different photosensitizers and differing light irradiation doses. Immortalized neuroblastoma (SHSY-5Y), proliferative human keratinocytes (HaCaT) and Glioblastoma Multiforme (U87MG) cell lines were exposed in vitro to progressively increasing 630nm laser irradiation, with the use of a first (5-Aminolevulinic Acid) and second-generation photosensitizer (Photofrin). Cell kill was assessed using a viability assay, quantified by a 96-well plate reader. Individually, neither irradiation, 5-aminolevulinic acid (ALA) or Photofrin caused death of U87MG, SHSY-5Y or HaCaT cells. However, when combining light irradiation with photosensitizers, both 5-ALA and Photofrin caused a dose-dependent reduction in the viability of U87MG glioblastoma cells, but the potency of light was higher in cells treated with Photofrin. SHSY-5Y neuroblastoma cells exhibited higher sensitivity to PDT (using 5-ALA) than U87MG and HaCat keratinocytes. SHSY-5Y and U87MG exhibited similar sensitivities to irradiation with Photofrin as the photosensitizer. Highly invasive tumours can demonstrate different behaviours when exposed to similar PDT doses with certain photosensitizers, while others produce a uniformity of response. This has strong implications for ongoing research, suggesting a mechanism beyond simple free radical generation, as well as the potential for those tumours exhibiting a poor response to PDT in prior work requiring investigation with other photosensitizers.

Original languageEnglish
Title of host publication17th International Photodynamic Association World Congress
EditorsTayyaba Hasan
PublisherSociety of Photo-optical Instrumentation Engineers
ISBN (Electronic)9781510628335
DOIs
Publication statusPublished - 7 Aug 2019
Event17th International Photodynamic Association World Congress 2019 - Cambridge, United States
Duration: 28 Jun 20194 Jul 2019

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume11070
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference17th International Photodynamic Association World Congress 2019
CountryUnited States
CityCambridge
Period28/06/194/07/19

Keywords

  • Glioblastoma Multiforme
  • High-grade tumours
  • Keratinocytes
  • Neuroblastoma
  • Photodynamic Therapy
  • Photosensitizers

Fingerprint Dive into the research topics of 'Determining an in vitro dose-response relationship of photodynamic therapy with first and second-generation photosensitisers for high grade tumours'. Together they form a unique fingerprint.

  • Cite this

    Singh, K., Baptista-Hon, D., Hewitt, M., Kouli, O., Hossain-Ibrahim, K., & Hales, T. (2019). Determining an in vitro dose-response relationship of photodynamic therapy with first and second-generation photosensitisers for high grade tumours. In T. Hasan (Ed.), 17th International Photodynamic Association World Congress [110706E] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 11070). Society of Photo-optical Instrumentation Engineers. https://doi.org/10.1117/12.2527574