Development of a doubly weighted Gerchberg-Saxton algorithm for use in multibeam imaging applications

Simon P. Poland, Nikola Krstajić, Robert D. Knight, Robert K. Henderson, Simon M. Ameer-Beg

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    We report on the development of a doubly weighted Gerchberg?Saxton algorithm (DWGS) to enable generation of uniform beamlet arrays with a spatial light modulator (SLM) for use in multiphoton multifocal imaging applications. The algorithm incorporates the WGS algorithm as well as feedback of fluorescence signals from the sample measured with a single-photon avalanche diode (SPAD) detector array. This technique compensates for issues associated with nonuniform illumination onto the SLM, the effects due to aberrations and the variability in gain between detectors within the SPAD array to generate a uniformly illuminated multiphoton fluorescence image. We demonstrate the use of the DWGS with a number of beamlet array patterns to image muscle fibers of a 5-day-old fixed zebrafish larvae.

    Original languageEnglish
    Pages (from-to)2431-2434
    Number of pages4
    JournalOptics Letters
    Volume39
    Issue number8
    DOIs
    Publication statusPublished - 15 Apr 2014

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