Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

J. Nylk; M. V. G. Kristensen; M. Mazilu; A. K. Thayil; C. A. Mitchell; E. C. Campbell; S. J. Powis; F. J. Gunn-Moore; K. Dholakia

doi:10.1364/BOE.6.001512

Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

J. Nylk (Lead / Corresponding author), M. V. G. Kristensen, M. Mazilu, A. K. Thayil, C. A. Mitchell, E. C. Campbell, S. J. Powis, F. J. Gunn-Moore, K. Dholakia

Physics

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

Original language	English
Pages (from-to)	1512-1519
Number of pages	8
Journal	Biomedical Optics Express
Volume	6
Issue number	4
Early online date	27 Mar 2015
DOIs	https://doi.org/10.1364/BOE.6.001512
Publication status	Published - 1 Apr 2015

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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abstract = "We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.",

author = "J. Nylk and Kristensen, {M. V. G.} and M. Mazilu and Thayil, {A. K.} and Mitchell, {C. A.} and Campbell, {E. C.} and Powis, {S. J.} and Gunn-Moore, {F. J.} and K. Dholakia",

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T1 - Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

AU - Nylk, J.

AU - Kristensen, M. V. G.

AU - Mazilu, M.

AU - Thayil, A. K.

AU - Mitchell, C. A.

AU - Campbell, E. C.

AU - Powis, S. J.

AU - Gunn-Moore, F. J.

AU - Dholakia, K.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

AB - We demonstrate a miniaturized single beam fiber optical trapping probe based on a high numerical aperture graded index (GRIN) micro-objective lens. This enables optical trapping at a distance of 200mm from the probe tip. The fiber trapping probe is characterized experimentally using power spectral density analysis and an original approach based on principal component analysis for accurate particle tracking. Its use for biomedical microscopy is demonstrated through optically mediated immunological synapse formation.

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U2 - 10.1364/BOE.6.001512

DO - 10.1364/BOE.6.001512

M3 - Article

AN - SCOPUS:84948771934

SN - 2156-7085

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SP - 1512

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JO - Biomedical Optics Express

JF - Biomedical Optics Express

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Development of a graded index microlens based fiber optical trap and its characterization using principal component analysis

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