Projects per year
Abstract
Although recently developed placenta-on-chip systems opened promising perspectives for placental barrier modeling, they still lack physiologically relevant trophoblasts and are poorly amenable to high-throughput studies. We aimed to implement human-induced pluripotent stem cells (hiPSC)-derived trophoblasts into a multi-well microfluidic device to develop a physiologically relevant and scalable placental barrier model. When cultured in a perfused micro-channel against a collagen-based matrix, hiPSC-derived trophoblasts self-arranged into a 3D structure showing invasive behavior, fusogenic and endocrine activities, structural integrity, and expressing placental transporters. RNA-seq analysis revealed that the microfluidic 3D environment boosted expression of genes related to early placental structural development, mainly involved in mechanosensing and cell surface receptor signaling. These results demonstrated the feasibility of generating a differentiated primitive syncytium from hiPSC in a microfluidic platform. Besides expanding hiPSC-derived trophoblast scope of applications, this study constitutes an important resource to improve placental barrier models and boost research and therapeutics evaluation in pregnancy.
Original language | English |
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Article number | 107240 |
Number of pages | 24 |
Journal | iScience |
Volume | 26 |
Issue number | 7 |
Early online date | 13 Jul 2023 |
DOIs | |
Publication status | Published - 21 Jul 2023 |
Keywords
- Biotechnology
- Cell biology
- Pathophysiology
ASJC Scopus subject areas
- General
Fingerprint
Dive into the research topics of 'Development of a human iPSC-derived placental barrier-on-chip model'. Together they form a unique fingerprint.Projects
- 3 Finished
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A Fully Integrated FLIM-FRET System For Imaging Dynamic Protein - Protein Interactions And Protein Turnover In Single Live Cells And Model Organisms
Appleton, P., Dinkova-Kostova, A., Hiom, K., Januschke, J., MacDonald, M., Saurin, A., Swedlow, J., Tanaka, T. & Weijer, K.
Biotechnology and Biological Sciences Research Council
1/11/20 → 31/03/21
Project: Research
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Investigating the Role of Thiol Modifications in Developing the Preeclampsia Phenotype
1/09/19 → 31/07/22
Project: Research
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Innovation in modelling Placenta for Maternal and Fetal Health (iPLACENTA) (joint with Aston University, Mimetas BV, St George's Medical School, University College Cork, Universita Degli Studi di Torino, Institut National de le Sante et de la Recherche Medicale, Universitaet Rostock, Fundacion Para le Investigacion del Hospital Universitario La Fe De La Comunidad Valencia, Katholieke Universiteit Leuven, Universiteit Maastricht)
COMMISSION OF THE EUROPEAN COMMUNITIES
1/03/18 → 31/05/22
Project: Research
Research output
- 1 Preprint
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Development of a Human iPSC-Derived Placental Barrier-On-Chip Model
Lermant, A., Rabussier, G., Lanz, H. L., Davidson, L., Porter, I. M. & Murdoch, C. E., 30 Jan 2023, Cell Press, (ISCIENCE-D-23-00286).Research output: Working paper/Preprint › Preprint
Press/Media
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Dundee scientists create world-first 'micro placenta'
14/07/23
1 Media contribution
Press/Media: Research
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Scottish scientists develop world-first ‘micro-placenta’
14/07/23
1 Media contribution
Press/Media: Research
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Dundee scientists in placenta stem cell breakthrough
14/07/23
1 Media contribution
Press/Media: Research