Development of a radioimmunoassay for the measurement of prostacyclin metabolites in unextracted plasma

M McLaren, J J F Belch, C D Forbes, C. R. M. Prentice

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    Abstract

    Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.
    Original languageEnglish
    Pages (from-to)177-183
    Number of pages7
    JournalThrombosis Research
    Volume37
    Issue number1
    DOIs
    Publication statusPublished - 1985

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