Development of a radioimmunoassay for the measurement of prostacyclin metabolites in unextracted plasma

M McLaren; J J F Belch; C D Forbes; C. R. M. Prentice

doi:10.1016/0049-3848(85)90044-1

Development of a radioimmunoassay for the measurement of prostacyclin metabolites in unextracted plasma

M McLaren, J J F Belch, C D Forbes, C. R. M. Prentice

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14 Citations (Scopus)

Abstract

Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.

Original language	English
Pages (from-to)	177-183
Number of pages	7
Journal	Thrombosis Research
Volume	37
Issue number	1
DOIs	https://doi.org/10.1016/0049-3848(85)90044-1
Publication status	Published - 1985

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abstract = "Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.",

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AU - McLaren, M

AU - Belch, J J F

AU - Forbes, C D

AU - Prentice, C. R. M.

PY - 1985

Y1 - 1985

N2 - Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.

AB - Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.

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DO - 10.1016/0049-3848(85)90044-1

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SP - 177

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JO - Thrombosis Research

JF - Thrombosis Research

IS - 1

ER -

Development of a radioimmunoassay for the measurement of prostacyclin metabolites in unextracted plasma

Abstract

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