Development of a radioimmunoassay for the measurement of prostacyclin metabolites in unextracted plasma

TY - JOUR

T1 - Development of a radioimmunoassay for the measurement of prostacyclin metabolites in unextracted plasma

AU - McLaren, M

AU - Belch, J J F

AU - Forbes, C D

AU - Prentice, C. R. M.

PY - 1985

Y1 - 1985

N2 - Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.

AB - Levels of plasma 6-keto-prostaglandin F1 alpha (6-keto-PGF 1 alpha) measured in normal subjects by radioimmunoassay show wide variation. In an attempt to develop a sensitive, specific and reproducible assay for the measurement of the circulating metabolites of prostacyclin (PGI2) we examined the different variables involved in radioimmunoassay such as choice of buffer; incubation time and temperature; amount of radioisotope tracer added and the separation method. The method described gives good reproducibility and shows good correlation with in vivo and in vitro doses of PGI2.

U2 - 10.1016/0049-3848(85)90044-1

DO - 10.1016/0049-3848(85)90044-1

M3 - Article

C2 - 3885471

SN - 0049-3848

VL - 37

SP - 177

EP - 183

JO - Thrombosis Research

JF - Thrombosis Research

IS - 1

ER -