Development of specific radioimmunoassays for the measurement of human hepatic basic and N/A2b glutathione S-transferases

G J Beckett, J D Hayes

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    A number of studies have indicated that plasma glutathione S-transferase (GST) measurements by radioimmunoassay (RIA) are superior to aminotransferase measurements at assessing hepatocellular damage [1,2]. In human liver three distinct groups of GST exist [3-S] and we have recently reported a purification scheme for GST in which three fractions of GST designated basic, N/Al and N/A2 were isolated 111. The basic fractions of GST could be further purified into five forms (a-c) which all shared immunological identity [1,3] and the N/A2 fraction could be further split into a minor component N/A2a and a major fraction N/AZb. Immunological studies showed that N/A2b and basic GST were immunologically distinct [l]. The published methods for measuring basic GST by RIA appear less sensitive than the corresponding methods described for the RIA of N/A2b GST [1,2,6]. These different sensitivities could be due to assay conditions or may merely reflect the quality of the antisera. This paper reports a detailed investigation into the RIA of basic (6 and E) and N/AZb GST, and describes assays with sufficient sensitivity to allow their measurement in serum taken from normal subjects and from patients with liver damage.
    Original languageEnglish
    Pages (from-to)267-73
    Number of pages7
    JournalClinica Chimica Acta
    Volume141
    Issue number2-3
    DOIs
    Publication statusPublished - 31 Aug 1984

    Keywords

    • Animals
    • Antibody Specificity
    • Cross Reactions
    • Glutathione Transferase/blood
    • Humans
    • Liver/enzymology
    • Liver Diseases/enzymology
    • Rabbits
    • Radioimmunoassay
    • Specimen Handling
    • Time Factors

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