Developmental profile of excitatory GABAA responses in cultured rat cerebellar granule cells

A. Cristina Rego; Jeremy J. Lambert; David G. Nicholls

doi:10.1097/00001756-200103050-00011

Developmental profile of excitatory GABA_A responses in cultured rat cerebellar granule cells

A. Cristina Rego
, Jeremy J. Lambert
, David G. Nicholls

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

GABA induced a transient increase in cytosolic free Ca²⁺ in cerebellar granule cells, which decreased from 3 to 8 days in vitro (DIV). Cytosolic Ca²⁺ changes induced by glutamate/glycine were comparable at 3 and 7 DIV. The GABA response was ascribed to GABA_A-receptor mediated depolarization activating L-type Ca²⁺ channels since the response was inhibited by bicuculline or nifedipine. GABA-mediated Ca²⁺ rise at 4 DIV was potentiated by pentobarbital or by the neurosteroid 5β-pregnan-3α-ol-20-one, or by decreasing the extracellular Cl^- concentration. Neurons cultured for > 7 DIV showed no rise in intracellular Ca²⁺ in response to GABA regardless of the Cl^- gradient. GABA_A receptor-mediated cytosolic Ca²⁺ rise suggests an important role for the excitatory activity of GABA in developing cerebellar granule neurons.

Original language	English
Pages (from-to)	477-482
Number of pages	6
Journal	NeuroReport
Volume	12
Issue number	3
DOIs	https://doi.org/10.1097/00001756-200103050-00011
Publication status	Published - 5 Mar 2001

Keywords

5β-Pregnan-3α-ol-20-one
Calcium
Cerebellar granule cells
GABA receptors
Mitochondria
Pentobarbital

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1097/00001756-200103050-00011

Cite this

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title = "Developmental profile of excitatory GABAA responses in cultured rat cerebellar granule cells",

abstract = "GABA induced a transient increase in cytosolic free Ca2+ in cerebellar granule cells, which decreased from 3 to 8 days in vitro (DIV). Cytosolic Ca2+ changes induced by glutamate/glycine were comparable at 3 and 7 DIV. The GABA response was ascribed to GABAA-receptor mediated depolarization activating L-type Ca2+ channels since the response was inhibited by bicuculline or nifedipine. GABA-mediated Ca2+ rise at 4 DIV was potentiated by pentobarbital or by the neurosteroid 5β-pregnan-3α-ol-20-one, or by decreasing the extracellular Cl- concentration. Neurons cultured for > 7 DIV showed no rise in intracellular Ca2+ in response to GABA regardless of the Cl- gradient. GABAA receptor-mediated cytosolic Ca2+ rise suggests an important role for the excitatory activity of GABA in developing cerebellar granule neurons.",

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AU - Rego, A. Cristina

AU - Lambert, Jeremy J.

AU - Nicholls, David G.

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Y1 - 2001/3/5

N2 - GABA induced a transient increase in cytosolic free Ca2+ in cerebellar granule cells, which decreased from 3 to 8 days in vitro (DIV). Cytosolic Ca2+ changes induced by glutamate/glycine were comparable at 3 and 7 DIV. The GABA response was ascribed to GABAA-receptor mediated depolarization activating L-type Ca2+ channels since the response was inhibited by bicuculline or nifedipine. GABA-mediated Ca2+ rise at 4 DIV was potentiated by pentobarbital or by the neurosteroid 5β-pregnan-3α-ol-20-one, or by decreasing the extracellular Cl- concentration. Neurons cultured for > 7 DIV showed no rise in intracellular Ca2+ in response to GABA regardless of the Cl- gradient. GABAA receptor-mediated cytosolic Ca2+ rise suggests an important role for the excitatory activity of GABA in developing cerebellar granule neurons.

AB - GABA induced a transient increase in cytosolic free Ca2+ in cerebellar granule cells, which decreased from 3 to 8 days in vitro (DIV). Cytosolic Ca2+ changes induced by glutamate/glycine were comparable at 3 and 7 DIV. The GABA response was ascribed to GABAA-receptor mediated depolarization activating L-type Ca2+ channels since the response was inhibited by bicuculline or nifedipine. GABA-mediated Ca2+ rise at 4 DIV was potentiated by pentobarbital or by the neurosteroid 5β-pregnan-3α-ol-20-one, or by decreasing the extracellular Cl- concentration. Neurons cultured for > 7 DIV showed no rise in intracellular Ca2+ in response to GABA regardless of the Cl- gradient. GABAA receptor-mediated cytosolic Ca2+ rise suggests an important role for the excitatory activity of GABA in developing cerebellar granule neurons.

KW - 5β-Pregnan-3α-ol-20-one

KW - Calcium

KW - Cerebellar granule cells

KW - GABA receptors

KW - Mitochondria

KW - Pentobarbital

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