Abstract
During brain development the duration of miniature inhibitory post synaptic currents (mIPSCs) mediated by GABAA receptors (GABAARs) progressively reduces, to accommodate the temporal demands required for precise network activity. Conventionally, this synaptic plasticity results from GABAAR subunit reorganisation. In particular, in certain developing neurones synaptic α2-GABAARs are replaced by α1-GABAARs. However, in thalamocortical neurones of the mouse ventrobasal (VB) thalamus, the major alteration to mIPSC kinetics occurs on post-natal (P) day 10, some days prior to the GABAAR isoform change. Here, whole-cell voltage-clamp recordings from VB neurones of mouse thalamic slices revealed that early in postnatal development (> P8), the mIPSC duration is prolonged by local neurosteroids acting in a paracrine, or autocrine manner to enhance GABAAR function. However, by P10, this neurosteroid 'tone' rapidly dissipates, thereby producing brief mIPSCs. This plasticity results from a lack of steroid substrate as pre-treatment of mature thalamic slices (P20-24) with the GABAAR-inactive precursor 5α-dihydroprogesterone (5α-DHP), resulted in markedly prolonged mIPSCs and a greatly enhanced tonic conductance, mediated by synaptic and extrasynaptic GABAARs respectively. In summary, endogenous neurosteroids profoundly influence GABA-ergic neurotransmission in developing VB neurones and govern a transition from slow to fast phasic synaptic events. Furthermore, the retained capacity for steroidogenesis in the mature thalamus raises the prospect that certain physiological, or pathophysiological conditions, may trigger neurosteroid neosynthesis, thereby providing a local mechanism for fine-tuning neuronal excitability. This article is protected by copyright. All rights reserved.
Original language | English |
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Pages (from-to) | 267-284 |
Number of pages | 37 |
Journal | Journal of Physiology |
Volume | 593 |
Issue number | 1 |
Early online date | 31 Oct 2014 |
DOIs | |
Publication status | Published - 1 Jan 2015 |
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Lambert, Jeremy
- Systems Medicine - Professor (Teaching and Research) of Neuropharmacology
Person: Academic