Dexamethasone and insulin activate serum and glucocorticoid-inducible kinase 1 (SGK1) via different molecular mechanisms in cortical collecting duct cells

Morag K. Mansley (Lead / Corresponding author), Gordon B. Watt, Sarah L. Francis, David J. Walker, Stephen C. Land, Matthew A. Bailey, Stuart M. Wilson

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    Abstract

    Serum and glucocorticoid-inducible kinase 1 (SGK1) is a protein kinase that contributes to the hormonal control of renal Na(+) retention by regulating the abundance of epithelial Na(+) channels (ENaC) at the apical surface of the principal cells of the cortical collecting duct (CCD). Although glucocorticoids and insulin stimulate Na(+) transport by activating SGK1, the responses follow different time courses suggesting that these hormones act by different mechanisms. We therefore explored the signaling pathways that allow dexamethasone and insulin to stimulate Na(+) transport in mouse CCD cells (mpkCCDcl4). Dexamethasone evoked a progressive augmentation of electrogenic Na(+) transport that became apparent after ~45 min latency and was associated with increases in SGK1 activity and abundance and with increased expression of SGK1 mRNA Although the catalytic activity of SGK1 is maintained by phosphatidylinositol-OH-3-kinase (PI3K), dexamethasone had no effect upon PI3K activity. Insulin also stimulated Na(+) transport but this response occurred with no discernible latency. Moreover, although insulin also activated SGK1, it had no effect upon SGK1 protein or mRNA abundance. Insulin did, however, evoke a clear increase in cellular PI3K activity. Our data are consistent with earlier work, which shows that glucocorticoids regulate Na(+) retention by inducing sgk1 gene expression, and also establish that this occurs independently of increased PI3K activity. Insulin, on the other hand, stimulates Na(+) transport via a mechanism independent of sgk1 gene expression that involves PI3K activation. Although both hormones act via SGK1, our data show that they activate this kinase by distinct physiological mechanisms.

    Original languageEnglish
    Article numbere12792
    Number of pages11
    JournalPhysiological Reports
    Volume4
    Issue number10
    DOIs
    Publication statusPublished - 24 May 2016

    Fingerprint

    Dexamethasone
    Glucocorticoids
    Phosphatidylinositol 3-Kinase
    Insulin
    serum-inducible kinase
    Hormones
    Epithelial Sodium Channels
    Gene Expression
    Messenger RNA
    Protein Kinases
    Phosphotransferases
    hydroxide ion
    Kidney

    Keywords

    • Cortical collecting duct
    • Epithelial Na channel
    • Na transport
    • Renal Na retention
    • Ussing chamber

    Cite this

    Mansley, Morag K. ; Watt, Gordon B. ; Francis, Sarah L. ; Walker, David J. ; Land, Stephen C. ; Bailey, Matthew A. ; Wilson, Stuart M. / Dexamethasone and insulin activate serum and glucocorticoid-inducible kinase 1 (SGK1) via different molecular mechanisms in cortical collecting duct cells. In: Physiological Reports. 2016 ; Vol. 4, No. 10.
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    abstract = "Serum and glucocorticoid-inducible kinase 1 (SGK1) is a protein kinase that contributes to the hormonal control of renal Na(+) retention by regulating the abundance of epithelial Na(+) channels (ENaC) at the apical surface of the principal cells of the cortical collecting duct (CCD). Although glucocorticoids and insulin stimulate Na(+) transport by activating SGK1, the responses follow different time courses suggesting that these hormones act by different mechanisms. We therefore explored the signaling pathways that allow dexamethasone and insulin to stimulate Na(+) transport in mouse CCD cells (mpkCCDcl4). Dexamethasone evoked a progressive augmentation of electrogenic Na(+) transport that became apparent after ~45 min latency and was associated with increases in SGK1 activity and abundance and with increased expression of SGK1 mRNA Although the catalytic activity of SGK1 is maintained by phosphatidylinositol-OH-3-kinase (PI3K), dexamethasone had no effect upon PI3K activity. Insulin also stimulated Na(+) transport but this response occurred with no discernible latency. Moreover, although insulin also activated SGK1, it had no effect upon SGK1 protein or mRNA abundance. Insulin did, however, evoke a clear increase in cellular PI3K activity. Our data are consistent with earlier work, which shows that glucocorticoids regulate Na(+) retention by inducing sgk1 gene expression, and also establish that this occurs independently of increased PI3K activity. Insulin, on the other hand, stimulates Na(+) transport via a mechanism independent of sgk1 gene expression that involves PI3K activation. Although both hormones act via SGK1, our data show that they activate this kinase by distinct physiological mechanisms.",
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    Dexamethasone and insulin activate serum and glucocorticoid-inducible kinase 1 (SGK1) via different molecular mechanisms in cortical collecting duct cells. / Mansley, Morag K. (Lead / Corresponding author); Watt, Gordon B.; Francis, Sarah L.; Walker, David J.; Land, Stephen C.; Bailey, Matthew A.; Wilson, Stuart M.

    In: Physiological Reports, Vol. 4, No. 10, e12792, 24.05.2016.

    Research output: Contribution to journalArticle

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    AU - Mansley, Morag K.

    AU - Watt, Gordon B.

    AU - Francis, Sarah L.

    AU - Walker, David J.

    AU - Land, Stephen C.

    AU - Bailey, Matthew A.

    AU - Wilson, Stuart M.

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    KW - Epithelial Na channel

    KW - Na transport

    KW - Renal Na retention

    KW - Ussing chamber

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