Dietary nucleotides and fecal microbiota in formula-fed infants: a randomized controlled trial

Atul Singhal (Lead / Corresponding author), George Macfarlane, Sandra Macfarlane, Julie Lanigan, Kathy Kennedy, Alun Elias-Jones, Terence Stephenson, Peter Dudek, Alan Lucas

    Research output: Contribution to journalArticlepeer-review

    73 Citations (Scopus)


    Background: Dietary nucleotides are nonprotein nitrogenous compounds that are thought to be important for growth, repair, and differentiation of the gastrointestinal tract. A higher nucleotide intake may also have favorable effects on the fecal microbial composition and incidence of diarrhea in infancy. However, few studies have tested this hypothesis with an experimental study design.

    Objective: We tested the hypothesis that nucleotide supplementation of infant formula has beneficial effects on fecal bacteriology.

    Design: Oligonucleotide probes were used to measure bacterial genus-specific 16S ribosomal RNA in stools of a subset of infants (mean age: 20.4 wk) who were randomly assigned to nucleotide-supplemented (31 mg/L; n = 35) or control formula (n = 37) from birth until age 20 wk or were breastfed (reference group; n = 44). The microbial pattern was assessed as the ratio of Bacteroides-Porphyromonas-Prevotella group (BPP) to Bifidobacterium species.

    Results: The ratio of BPP to Bifidobacterium spp. rRNA in infants randomly assigned to the nucleotide-supplemented formula was lower than in infants receiving the control formula (mean difference: -118%; 95% CI: -203%, -34%; P = 0.007), but it did not differ in infants who were breastfed. The difference between randomized formula-fed groups was independent of potential confounding factors (P = 0.003).

    Conclusions: Our data support the hypothesis that nucleotide supplementation improves the composition of the gut microbiota in formula-fed infants. Because this effect could contribute to previously described benefits of nucleotide supplementation for gastrointestinal tract and immune function, these findings have important implications for optimizing the diet of formula-fed infants.

    Original languageEnglish
    Pages (from-to)1785-1792
    Number of pages8
    JournalAmerican Journal of Clinical Nutrition
    Issue number6
    Publication statusPublished - 1 Jun 2008


    • Oligonucleotide probe
    • Diarrheal disease
    • Human milk
    • Flora
    • Ribonucleodites
    • Supplementation
    • Bifidobacterium
    • Quantification
    • Identification
    • Hybridization


    Dive into the research topics of 'Dietary nucleotides and fecal microbiota in formula-fed infants: a randomized controlled trial'. Together they form a unique fingerprint.

    Cite this