@article{1417e184a39247458cdc522dac4c3697,
title = "Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides",
abstract = "The major nutrients available to human colonic Bacteroides species are glycans, exemplified by pectins, a network of covalently linked plant cell wall polysaccharides containing galacturonic acid (GalA). Metabolism of complex carbohydrates by the Bacteroides genus is orchestrated by polysaccharide utilization loci (PULs). In Bacteroides thetaiotaomicron, a human colonic bacterium, the PULs activated by different pectin domains have been identified; however, the mechanism by which these loci contribute to the degradation of these GalA-containing polysaccharides is poorly understood. Here we show that each PUL orchestrates the metabolism of specific pectin molecules, recruiting enzymes from two previously unknown glycoside hydrolase families. The apparatus that depolymerizes the backbone of rhamnogalacturonan-I is particularly complex. This system contains several glycoside hydrolases that trim the remnants of other pectin domains attached to rhamnogalacturonan-I, and nine enzymes that contribute to the degradation of the backbone that makes up a rhamnose-GalA repeating unit. The catalytic properties of the pectin-degrading enzymes are optimized to protect the glycan cues that activate the specific PULs ensuring a continuous supply of inducing molecules throughout growth. The contribution of Bacteroides spp. to metabolism of the pectic network is illustrated by cross-feeding between organisms.",
author = "Luis, {Ana S.} and Jonathon Briggs and Xiaoyang Zhang and Benjamin Farnell and Didier Ndeh and Aurore Labourel and Arnaud Basl{\'e} and Alan Cartmell and Nicolas Terrapon and Katherine Stott and Lowe, {Elisabeth C.} and Richard McLean and Kaitlyn Shearer and Julia Sch{\"u}ckel and Immacolata Venditto and Ralet, {Marie Christine} and Bernard Henrissat and Martens, {Eric C.} and Mosimann, {Steven C.} and Abbott, {D. Wade} and Gilbert, {Harry J.}",
note = "Funding Information: This work was supported in part by an Advanced Grant from the European Research Council (Grant No. 322820) awarded to H.J.G. and B.H. supporting A.S.L., D.N., A.C. and N.T., a Wellcome Trust Senior Investigator Award to H.J.G. (grant No. WT097907MA) that supported J.B. and E.C.L. a European Union Seventh Framework Initial Training Network Programme entitled the “WallTraC project” (Grant Agreement number 263916) awarded to M-C.R. and H.J.G, which supported X.Z. and J.S. The Biotechnology and Biological Research Council project {\textquoteleft}Ricefuel{\textquoteright} (grant numbers BB/K020358/1) awarded to H.J.G. supported A.L. We thank Diamond Light Source for access to beamline I02, I04-1 and I24 (mx1960, mx7854 and mx9948) that contributed to the results presented here, and to J. Gray at Newcastle University for assistance with the mass spectrometry. Publisher Copyright: {\textcopyright} 2017 The Author(s). Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2018",
month = feb,
day = "1",
doi = "10.1038/s41564-017-0079-1",
language = "English",
volume = "3",
pages = "210--219",
journal = "Nature Microbiology",
issn = "2058-5276",
publisher = "Nature Publishing Group",
number = "2",
}