Differences in the frequency of macrophage and t cell markers between focal and crescentic classes of antineutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis

Dana Kidder (Lead / Corresponding author), Susan E. Bray, Stewart Fleming

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Abstract

Background: Anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis (AAGN) can be classified into; focal, crescentic, mixed and sclerotic classes. Macrophages and T lymphocytes are key players in mediating renal injury. The frequency of macrophage and T lymphocytes in different histological classes is unclear. 

Objectives: We examined the frequency of macrophage and T lymphocyte markers in AAGN and assessed their correlation with renal function at presentation.

Patients and Methods: Renal biopsies from 38 patients were included in immunohistochemistry analysis of macrophages (CD68, sialoadhesin [Sn] and mannose receptor [MR]) and T cells (CD4 and CD8) markers. The frequency of these markers in glomerular, periglomerular and interstitial compartments were measured in a blinded fashion. Biopsies were allocated a histological class of focal, crescentic, mixed or sclerotic. Scores were then matched to histological class and assessed for correlation with renal function.

Results: The biopsies were crescentic 19 (50%), focal 10 (26.3%), mixed 6 (15.7%) and sclerotic 3 (8%). Interstitial CD68+ macrophages and CD8+ T lymphocytes showed best correlation with renal function at the time of presentation. CD68+ macrophages were significantly increased in crescentic compared to focal AAGN. MR+ macrophages, CD4 and CD8 T cells were also elevated in the interstitium of crescentic compared to focal group.

Conclusions: In this study interstitial CD68 and CD8 showed the highest association with the renal function at presentation. Differences in the cellular infiltrate between focal and crescentic AAGN were related to CD68+ macrophages and to interstitial MR+ macrophages and T lymphocytes. Further studies are needed to assess these differences across all four histological categories.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalJournal of Nephropathology
Volume6
Issue number2
Early online date25 Dec 2016
DOIs
Publication statusPublished - 2017

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Antineutrophil Cytoplasmic Antibodies
Glomerulonephritis
Macrophages
T-Lymphocytes
Kidney
Biopsy
Sialic Acid Binding Ig-like Lectin 1
Immunohistochemistry

Keywords

  • Anti-neutrophil cytoplasmic antibody
  • Glomerulonephritis
  • Macrophage
  • T lymphocyte

Cite this

@article{c59c5b120ddd4cd080ebd71b97f721ba,
title = "Differences in the frequency of macrophage and t cell markers between focal and crescentic classes of antineutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis",
abstract = "Background: Anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis (AAGN) can be classified into; focal, crescentic, mixed and sclerotic classes. Macrophages and T lymphocytes are key players in mediating renal injury. The frequency of macrophage and T lymphocytes in different histological classes is unclear. Objectives: We examined the frequency of macrophage and T lymphocyte markers in AAGN and assessed their correlation with renal function at presentation.Patients and Methods: Renal biopsies from 38 patients were included in immunohistochemistry analysis of macrophages (CD68, sialoadhesin [Sn] and mannose receptor [MR]) and T cells (CD4 and CD8) markers. The frequency of these markers in glomerular, periglomerular and interstitial compartments were measured in a blinded fashion. Biopsies were allocated a histological class of focal, crescentic, mixed or sclerotic. Scores were then matched to histological class and assessed for correlation with renal function.Results: The biopsies were crescentic 19 (50{\%}), focal 10 (26.3{\%}), mixed 6 (15.7{\%}) and sclerotic 3 (8{\%}). Interstitial CD68+ macrophages and CD8+ T lymphocytes showed best correlation with renal function at the time of presentation. CD68+ macrophages were significantly increased in crescentic compared to focal AAGN. MR+ macrophages, CD4 and CD8 T cells were also elevated in the interstitium of crescentic compared to focal group.Conclusions: In this study interstitial CD68 and CD8 showed the highest association with the renal function at presentation. Differences in the cellular infiltrate between focal and crescentic AAGN were related to CD68+ macrophages and to interstitial MR+ macrophages and T lymphocytes. Further studies are needed to assess these differences across all four histological categories.",
keywords = "Anti-neutrophil cytoplasmic antibody, Glomerulonephritis, Macrophage, T lymphocyte",
author = "Dana Kidder and Bray, {Susan E.} and Stewart Fleming",
year = "2017",
doi = "10.15171/jnp.2017.16",
language = "English",
volume = "6",
pages = "97--102",
journal = "Journal of Nephropathology",
issn = "2251-8363",
publisher = "Society of Diabetic Nephropathy Prevention",
number = "2",

}

TY - JOUR

T1 - Differences in the frequency of macrophage and t cell markers between focal and crescentic classes of antineutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis

AU - Kidder, Dana

AU - Bray, Susan E.

AU - Fleming, Stewart

PY - 2017

Y1 - 2017

N2 - Background: Anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis (AAGN) can be classified into; focal, crescentic, mixed and sclerotic classes. Macrophages and T lymphocytes are key players in mediating renal injury. The frequency of macrophage and T lymphocytes in different histological classes is unclear. Objectives: We examined the frequency of macrophage and T lymphocyte markers in AAGN and assessed their correlation with renal function at presentation.Patients and Methods: Renal biopsies from 38 patients were included in immunohistochemistry analysis of macrophages (CD68, sialoadhesin [Sn] and mannose receptor [MR]) and T cells (CD4 and CD8) markers. The frequency of these markers in glomerular, periglomerular and interstitial compartments were measured in a blinded fashion. Biopsies were allocated a histological class of focal, crescentic, mixed or sclerotic. Scores were then matched to histological class and assessed for correlation with renal function.Results: The biopsies were crescentic 19 (50%), focal 10 (26.3%), mixed 6 (15.7%) and sclerotic 3 (8%). Interstitial CD68+ macrophages and CD8+ T lymphocytes showed best correlation with renal function at the time of presentation. CD68+ macrophages were significantly increased in crescentic compared to focal AAGN. MR+ macrophages, CD4 and CD8 T cells were also elevated in the interstitium of crescentic compared to focal group.Conclusions: In this study interstitial CD68 and CD8 showed the highest association with the renal function at presentation. Differences in the cellular infiltrate between focal and crescentic AAGN were related to CD68+ macrophages and to interstitial MR+ macrophages and T lymphocytes. Further studies are needed to assess these differences across all four histological categories.

AB - Background: Anti-neutrophil cytoplasmic antibody (ANCA)-associated glomerulonephritis (AAGN) can be classified into; focal, crescentic, mixed and sclerotic classes. Macrophages and T lymphocytes are key players in mediating renal injury. The frequency of macrophage and T lymphocytes in different histological classes is unclear. Objectives: We examined the frequency of macrophage and T lymphocyte markers in AAGN and assessed their correlation with renal function at presentation.Patients and Methods: Renal biopsies from 38 patients were included in immunohistochemistry analysis of macrophages (CD68, sialoadhesin [Sn] and mannose receptor [MR]) and T cells (CD4 and CD8) markers. The frequency of these markers in glomerular, periglomerular and interstitial compartments were measured in a blinded fashion. Biopsies were allocated a histological class of focal, crescentic, mixed or sclerotic. Scores were then matched to histological class and assessed for correlation with renal function.Results: The biopsies were crescentic 19 (50%), focal 10 (26.3%), mixed 6 (15.7%) and sclerotic 3 (8%). Interstitial CD68+ macrophages and CD8+ T lymphocytes showed best correlation with renal function at the time of presentation. CD68+ macrophages were significantly increased in crescentic compared to focal AAGN. MR+ macrophages, CD4 and CD8 T cells were also elevated in the interstitium of crescentic compared to focal group.Conclusions: In this study interstitial CD68 and CD8 showed the highest association with the renal function at presentation. Differences in the cellular infiltrate between focal and crescentic AAGN were related to CD68+ macrophages and to interstitial MR+ macrophages and T lymphocytes. Further studies are needed to assess these differences across all four histological categories.

KW - Anti-neutrophil cytoplasmic antibody

KW - Glomerulonephritis

KW - Macrophage

KW - T lymphocyte

UR - http://www.nephropathol.com/Abstract/JNP_20170102201232

UR - http://www.scopus.com/inward/record.url?scp=85008466266&partnerID=8YFLogxK

U2 - 10.15171/jnp.2017.16

DO - 10.15171/jnp.2017.16

M3 - Article

C2 - 28491860

AN - SCOPUS:85008466266

VL - 6

SP - 97

EP - 102

JO - Journal of Nephropathology

JF - Journal of Nephropathology

SN - 2251-8363

IS - 2

ER -