Different cellular response mechanisms contribute to the length-dependent cytotoxicity of multi-walled carbon nanotubes

Dun Liu, Lijun Wang, Zhigang Wang, Alfred Cuschieri

    Research output: Contribution to journalArticle

    39 Citations (Scopus)

    Abstract

    To date, there has not been an agreement on the best methods for the characterisation of multi-walled carbon nanotube (MWCNT) toxicity. The length of MWCNTs has been identified as a factor in in vitro and in vivo studies, in addition to their purity and biocompatible coating. Another unresolved issue relates to the variable toxicity of MWCNTs on different cell types. The present study addressed the effects of MWCNTs' length on mammalian immune and epithelial cancer cells RAW264.7 and MCF-7, respectively. Our data confirm that MWCNTs induce cytotoxicity in a length- and cell type-dependent manner. Whereas, longer (3 to 14 µm) MWCNTs exert high toxicity, especially to RAW264.7 cells, shorter (1.5 µm) MWCNTs are significantly less cytotoxic. These findings confirm that the degree of biocompatibility of MWCNTs is closely related to their length and that immune cells appear to be more susceptible to damage by MWCNTs. Our study also indicates that MWCNT nanotoxicity should be analysed for various components of cellular response, and cytotoxicity data should be validated by the use of more than one assay system. Results from chromogenic-based assays should be confirmed by trypan blue exclusion.
    Original languageEnglish
    Article number361
    Pages (from-to)1-21
    Number of pages21
    JournalNanoscale Research Letters
    Volume7
    DOIs
    Publication statusPublished - 2012

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    Carbon Nanotubes
    Cytotoxicity
    Toxicity
    Carbon nanotubes
    carbon nanotubes
    toxicity
    Assays
    cells
    Chromogenics
    Trypan Blue
    Biocompatibility
    biocompatibility
    exclusion
    Cells
    Coatings
    purity
    cancer
    damage
    coatings

    Cite this

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    abstract = "To date, there has not been an agreement on the best methods for the characterisation of multi-walled carbon nanotube (MWCNT) toxicity. The length of MWCNTs has been identified as a factor in in vitro and in vivo studies, in addition to their purity and biocompatible coating. Another unresolved issue relates to the variable toxicity of MWCNTs on different cell types. The present study addressed the effects of MWCNTs' length on mammalian immune and epithelial cancer cells RAW264.7 and MCF-7, respectively. Our data confirm that MWCNTs induce cytotoxicity in a length- and cell type-dependent manner. Whereas, longer (3 to 14 µm) MWCNTs exert high toxicity, especially to RAW264.7 cells, shorter (1.5 µm) MWCNTs are significantly less cytotoxic. These findings confirm that the degree of biocompatibility of MWCNTs is closely related to their length and that immune cells appear to be more susceptible to damage by MWCNTs. Our study also indicates that MWCNT nanotoxicity should be analysed for various components of cellular response, and cytotoxicity data should be validated by the use of more than one assay system. Results from chromogenic-based assays should be confirmed by trypan blue exclusion.",
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    Different cellular response mechanisms contribute to the length-dependent cytotoxicity of multi-walled carbon nanotubes. / Liu, Dun; Wang, Lijun; Wang, Zhigang; Cuschieri, Alfred.

    In: Nanoscale Research Letters, Vol. 7, 361, 2012, p. 1-21.

    Research output: Contribution to journalArticle

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