Different routes for integral protein insertion into Ricinus communis protein-body and glyoxysome membranes

Claire Halpin, Michael J. Conder, J. Michael Lord

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Total polyadenylated RNA from ripening or germinating Ricinus communis L. endosperm was translated in rabbit reticulocyte lysate in the absence or presence of canine pancreatic microsomes. The products were immunoprecipitated using antibodies raised againts Triton X-114-extracted integral membrane proteins of protein bodies or glyoxysomes. While the proteins of proteinbody membranes were found to insert co-translationally into added microsomes, this was not observed in the case of glyoxysomal proteins. This observation was confirmed using antibodies raised against a purified glyoxysome membrane protein, alkaline lipase. These results indicate that different routes exist for the insertion of membrane proteins into the two organelles. In both cases membrane-protein insertion does not appear to be accompanied by proteolytic processing.

Original languageEnglish
Pages (from-to)331-339
Number of pages9
JournalPlanta
Volume179
Issue number3
DOIs
Publication statusPublished - Oct 1989

Keywords

  • Glyoxysome
  • Membrane protein (insertion)
  • Protein body
  • Ricinus (membrane proteins)

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