Differential antibody binding to the surface αβTCR·CD3 complex of CD4+ and CD8+ T lymphocytes is conserved in mammals and associated with differential glycosylation

Nineth E. Rossi, Jesús Reiné, Miguel Pineda-Lezamit, Manuel Pulgar, Néstor W. Meza, Mahima Swamy, Ruth Risueno, Wolfgang W.A. Schamel, Pedro Bonay, Edgar Fernández-Malavé, José R. Regueiro (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

We have previously shown that the surface αβ T cell antigen receptor (TCR)·CD3 complex borne by human CD4+ and CD8+ T lymphocytes can be distinguished using mAbs. Using two unrelated sets of antibodies, we have now extended this finding to the surface αβTCR·CD3 of seven additional mammalian species (six non-human primates and the mouse). We have also produced data supporting that differential glycosylation of the two main T cell subsets is involved in the observed TCR·CD3 antibody-binding differences in humans. First, we show differential lectin binding to human CD4+ versus CD8+ T lymphocytes, particularly with galectin 7. Second, we show that certain lectins can compete differentially with CD3 mAb binding to human primary CD4+ and CD8+ T lymphocytes. Third, N-glycan disruption using swainsonine was shown to increase mAb binding to the αβTCR·CD3. We conclude that the differential antibody binding to the surface αβTCR·CD3 complex of primary CD4+ and CD8+ T lymphocytes is phylogenetically conserved and associated with differential glycosylation. The differences may be exploited for therapeutic purposes, such as T cell lineage-specific immunosuppression of graft rejection. Also, the impact of glycosylation on CD3 antibody binding requires a cautious interpretation of CD3 expression levels and T cell numbers in clinical diagnosis.

Original languageEnglish
Pages (from-to)1247-1258
Number of pages12
JournalInternational Immunology
Volume20
Issue number10
DOIs
Publication statusPublished - 6 Oct 2008

Keywords

  • Cell surface molecules
  • T cells
  • TCRs

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