Differential Expression and Regulation by Activin of the Neurotrophins BDNF and NT4 During Human and Mouse Ovarian Development

Andrew J. Childs, Rosemary A. L. Bayne, Alison A. Murray, Sarah J. Martins Da Silva, Craig S. Collins, Norah Spears, Richard A. Anderson

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    The tropomyosin-related kinase (Trk) B neurotrophin receptor is essential for ovarian germ cell survival and primordial follicle formation, but the contributions of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4), are unknown. We have investigated their expression and regulation in developing human and mouse ovaries. BDNF expression increased with increasing gestation, expression of human NTF4 and of both Ntf5 and Bdnf in the mouse was unchanged. Bdnf expression was dramatically lower than Ntf5 in the mouse, but levels were comparable in the human. Human fetal ovarian somatic cells expressed BDNF. Activin A selectively regulated BDNF and Ntf5 expression in human and mouse, respectively, identifying an oocyte/somatic signaling pathway which might mediate the pro-survival effects of activin. These data reveal that expression and regulation of the TrkB ligands are differentially controlled in the developing ovaries of humans and mice, and identify BDNF as a potential regulator of germ cell fate in the human fetal ovary. Developmental Dynamics 239:1211-1219, 2010. (C) 2010 Wiley-Liss, Inc.

    Original languageEnglish
    Pages (from-to)1211-1219
    Number of pages9
    JournalDevelopmental Dynamics
    Volume239
    Issue number4
    DOIs
    Publication statusPublished - Apr 2010

    Keywords

    • neurotrophin
    • BDNF
    • NT4
    • ovary
    • oocyte
    • primordial follicle
    • activin
    • PRIMORDIAL FOLLICLE FORMATION
    • GERM-CELL SURVIVAL
    • HUMAN FETAL TESTIS
    • GENE-EXPRESSION
    • MAMMALIAN OVARY
    • RECEPTORS
    • NEURONS
    • GROWTH
    • MICE
    • FOLLICULOGENESIS

    Cite this

    Childs, Andrew J. ; Bayne, Rosemary A. L. ; Murray, Alison A. ; Martins Da Silva, Sarah J. ; Collins, Craig S. ; Spears, Norah ; Anderson, Richard A. / Differential Expression and Regulation by Activin of the Neurotrophins BDNF and NT4 During Human and Mouse Ovarian Development. In: Developmental Dynamics. 2010 ; Vol. 239, No. 4. pp. 1211-1219.
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    abstract = "The tropomyosin-related kinase (Trk) B neurotrophin receptor is essential for ovarian germ cell survival and primordial follicle formation, but the contributions of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4), are unknown. We have investigated their expression and regulation in developing human and mouse ovaries. BDNF expression increased with increasing gestation, expression of human NTF4 and of both Ntf5 and Bdnf in the mouse was unchanged. Bdnf expression was dramatically lower than Ntf5 in the mouse, but levels were comparable in the human. Human fetal ovarian somatic cells expressed BDNF. Activin A selectively regulated BDNF and Ntf5 expression in human and mouse, respectively, identifying an oocyte/somatic signaling pathway which might mediate the pro-survival effects of activin. These data reveal that expression and regulation of the TrkB ligands are differentially controlled in the developing ovaries of humans and mice, and identify BDNF as a potential regulator of germ cell fate in the human fetal ovary. Developmental Dynamics 239:1211-1219, 2010. (C) 2010 Wiley-Liss, Inc.",
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    author = "Childs, {Andrew J.} and Bayne, {Rosemary A. L.} and Murray, {Alison A.} and {Martins Da Silva}, {Sarah J.} and Collins, {Craig S.} and Norah Spears and Anderson, {Richard A.}",
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    Differential Expression and Regulation by Activin of the Neurotrophins BDNF and NT4 During Human and Mouse Ovarian Development. / Childs, Andrew J.; Bayne, Rosemary A. L.; Murray, Alison A.; Martins Da Silva, Sarah J.; Collins, Craig S.; Spears, Norah; Anderson, Richard A.

    In: Developmental Dynamics, Vol. 239, No. 4, 04.2010, p. 1211-1219.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Differential Expression and Regulation by Activin of the Neurotrophins BDNF and NT4 During Human and Mouse Ovarian Development

    AU - Childs, Andrew J.

    AU - Bayne, Rosemary A. L.

    AU - Murray, Alison A.

    AU - Martins Da Silva, Sarah J.

    AU - Collins, Craig S.

    AU - Spears, Norah

    AU - Anderson, Richard A.

    PY - 2010/4

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    N2 - The tropomyosin-related kinase (Trk) B neurotrophin receptor is essential for ovarian germ cell survival and primordial follicle formation, but the contributions of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4), are unknown. We have investigated their expression and regulation in developing human and mouse ovaries. BDNF expression increased with increasing gestation, expression of human NTF4 and of both Ntf5 and Bdnf in the mouse was unchanged. Bdnf expression was dramatically lower than Ntf5 in the mouse, but levels were comparable in the human. Human fetal ovarian somatic cells expressed BDNF. Activin A selectively regulated BDNF and Ntf5 expression in human and mouse, respectively, identifying an oocyte/somatic signaling pathway which might mediate the pro-survival effects of activin. These data reveal that expression and regulation of the TrkB ligands are differentially controlled in the developing ovaries of humans and mice, and identify BDNF as a potential regulator of germ cell fate in the human fetal ovary. Developmental Dynamics 239:1211-1219, 2010. (C) 2010 Wiley-Liss, Inc.

    AB - The tropomyosin-related kinase (Trk) B neurotrophin receptor is essential for ovarian germ cell survival and primordial follicle formation, but the contributions of its ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NT4), are unknown. We have investigated their expression and regulation in developing human and mouse ovaries. BDNF expression increased with increasing gestation, expression of human NTF4 and of both Ntf5 and Bdnf in the mouse was unchanged. Bdnf expression was dramatically lower than Ntf5 in the mouse, but levels were comparable in the human. Human fetal ovarian somatic cells expressed BDNF. Activin A selectively regulated BDNF and Ntf5 expression in human and mouse, respectively, identifying an oocyte/somatic signaling pathway which might mediate the pro-survival effects of activin. These data reveal that expression and regulation of the TrkB ligands are differentially controlled in the developing ovaries of humans and mice, and identify BDNF as a potential regulator of germ cell fate in the human fetal ovary. Developmental Dynamics 239:1211-1219, 2010. (C) 2010 Wiley-Liss, Inc.

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    KW - GERM-CELL SURVIVAL

    KW - HUMAN FETAL TESTIS

    KW - GENE-EXPRESSION

    KW - MAMMALIAN OVARY

    KW - RECEPTORS

    KW - NEURONS

    KW - GROWTH

    KW - MICE

    KW - FOLLICULOGENESIS

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