Dimethyl phenyl piperazine iodide (DMPP) induces glioma regression by inhibiting angiogenesis

Yan-qing He, Yan Li, Xiao-yu Wang, Xiao-dong He, Li Jun, Manli Chuai, Kenneth Ka Ho Lee, Ju Wang, Li-jing Wang (Lead / Corresponding author), Xuesong Yang (Lead / Corresponding author)

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    17 Citations (Scopus)

    Abstract

    1,1-Dimethyl-4-phenyl piperazine iodide (DMPP) is a synthetic nicotinic acetylcholine receptor (nAChR) agonist that could reduce airway inflammation. In this study, we demonstrated that DMPP could dramatically inhibit glioma size maintained on the chick embryonic chorioallantoic membrane (CAM). We first performed MTT and BrdU incorporation experiments on U87 glioma cells in vitro to understand the mechanism involved. We established that DMPP did not significantly affect U87 cell proliferation and survival. We speculated that DMPP directly caused the tumor to regress by affecting the vasculature in and around the implanted tumor on our chick CAM model. Hence, we conducted detailed analysis of DMPP's inhibitory effects on angiogenesis. Three vasculogenesis and angiogenesis in vivo models were used in the study which included (1) early chick blood islands formation, (2) chick yolk-sac membrane (YSW) and (3) CAM models. The results revealed that DMPP directly suppressed all developmental stages involved in vasculogenesis and angiogenesis - possibly by acting through Ang-1 and HIF-2. a signaling. In sum, our results show that DMPP could induce glioma regression grown on CAM by inhibiting vasculogenesis and angiogenesis.
    Original languageEnglish
    Pages (from-to)354-364
    Number of pages11
    JournalExperimental Cell Research
    Volume320
    Issue number2
    DOIs
    Publication statusPublished - 15 Jan 2014

    Keywords

    • DMPP
    • Vasculogenesis
    • Angiogenesis
    • YSM
    • CAM
    • Blood islands

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  • Cite this

    He, Y., Li, Y., Wang, X., He, X., Jun, L., Chuai, M., Lee, K. K. H., Wang, J., Wang, L., & Yang, X. (2014). Dimethyl phenyl piperazine iodide (DMPP) induces glioma regression by inhibiting angiogenesis. Experimental Cell Research, 320(2), 354-364. https://doi.org/10.1016/j.yexcr.2013.10.009