Direct and random routing of a molecular motor protein at a DNA junction

Louise K. Stanley, Mark D. Szczelkun (Lead / Corresponding author)

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)
    4 Downloads (Pure)


    With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3′-5′ double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks.

    Original languageEnglish
    Pages (from-to)4387-4394
    Number of pages8
    JournalNucleic Acids Research
    Issue number16
    Early online date26 Aug 2006
    Publication statusPublished - 1 Sept 2006

    ASJC Scopus subject areas

    • Genetics


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