Direct demonstration of sulphonylurea-sensitive KATP channels on nerve terminals of the rat motor cortex

Kevin Lee; Alistair K Dixon; Iain C. M. Rowe; Michael L. J. Ashford; Peter J. Richardson

Direct demonstration of sulphonylurea-sensitive KATP channels on nerve terminals of the rat motor cortex

Kevin Lee, Alistair K Dixon, Iain C. M. Rowe, Michael L. J. Ashford, Peter J. Richardson

Research output: Contribution to journal › Article › peer-review

Abstract

We examined whether ATP-sensitive potassium (KATP) channels are present on presynaptic terminals of the rat motor cortex, an area of the CNS exhibiting a high density of sulphonylurea binding. A novel fused nerve terminal preparation was developed which produced structures amenable to patch clamp methods. In inside-out recordings a K+ channel was observed which possessed all the major features of the Type 1 KATP channel, including sensitivity to ATP and the antidiabetic sulphonylureas.

Original language	English
Pages (from-to)	385-7
Number of pages	3
Journal	British Journal of Pharmacology
Volume	115
Issue number	3
Publication status	Published - Jun 1995

Keywords

Animals
Acetylcholinesterase
Sulfonylurea Compounds
Presynaptic Terminals
Citrate (si)-Synthase
Electric Stimulation
Potassium Channels
Binding Sites
Rats
Cerebral Cortex
L-Lactate Dehydrogenase
Rats, Sprague-Dawley
Patch-Clamp Techniques
Proteins
Nerve Endings
Adenosine Triphosphate
Choline O-Acetyltransferase
Male

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1908424/

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title = "Direct demonstration of sulphonylurea-sensitive KATP channels on nerve terminals of the rat motor cortex",

abstract = "We examined whether ATP-sensitive potassium (KATP) channels are present on presynaptic terminals of the rat motor cortex, an area of the CNS exhibiting a high density of sulphonylurea binding. A novel fused nerve terminal preparation was developed which produced structures amenable to patch clamp methods. In inside-out recordings a K+ channel was observed which possessed all the major features of the Type 1 KATP channel, including sensitivity to ATP and the antidiabetic sulphonylureas.",

keywords = "Animals, Acetylcholinesterase, Sulfonylurea Compounds, Presynaptic Terminals, Citrate (si)-Synthase, Electric Stimulation, Potassium Channels, Binding Sites, Rats, Cerebral Cortex, L-Lactate Dehydrogenase, Rats, Sprague-Dawley, Patch-Clamp Techniques, Proteins, Nerve Endings, Adenosine Triphosphate, Choline O-Acetyltransferase, Male",

author = "Kevin Lee and Dixon, {Alistair K} and Rowe, {Iain C. M.} and Ashford, {Michael L. J.} and Richardson, {Peter J.}",

year = "1995",

month = jun,

language = "English",

volume = "115",

pages = "385--7",

journal = "British Journal of Pharmacology",

issn = "0007-1188",

publisher = "Wiley",

number = "3",

}

TY - JOUR

T1 - Direct demonstration of sulphonylurea-sensitive KATP channels on nerve terminals of the rat motor cortex

AU - Lee, Kevin

AU - Dixon, Alistair K

AU - Rowe, Iain C. M.

AU - Ashford, Michael L. J.

AU - Richardson, Peter J.

PY - 1995/6

Y1 - 1995/6

N2 - We examined whether ATP-sensitive potassium (KATP) channels are present on presynaptic terminals of the rat motor cortex, an area of the CNS exhibiting a high density of sulphonylurea binding. A novel fused nerve terminal preparation was developed which produced structures amenable to patch clamp methods. In inside-out recordings a K+ channel was observed which possessed all the major features of the Type 1 KATP channel, including sensitivity to ATP and the antidiabetic sulphonylureas.

AB - We examined whether ATP-sensitive potassium (KATP) channels are present on presynaptic terminals of the rat motor cortex, an area of the CNS exhibiting a high density of sulphonylurea binding. A novel fused nerve terminal preparation was developed which produced structures amenable to patch clamp methods. In inside-out recordings a K+ channel was observed which possessed all the major features of the Type 1 KATP channel, including sensitivity to ATP and the antidiabetic sulphonylureas.

KW - Animals

KW - Acetylcholinesterase

KW - Sulfonylurea Compounds

KW - Presynaptic Terminals

KW - Citrate (si)-Synthase

KW - Electric Stimulation

KW - Potassium Channels

KW - Binding Sites

KW - Rats

KW - Cerebral Cortex

KW - L-Lactate Dehydrogenase

KW - Rats, Sprague-Dawley

KW - Patch-Clamp Techniques

KW - Proteins

KW - Nerve Endings

KW - Adenosine Triphosphate

KW - Choline O-Acetyltransferase

KW - Male

M3 - Article

C2 - 7582446

SN - 0007-1188

VL - 115

SP - 385

EP - 387

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

IS - 3

ER -

Direct demonstration of sulphonylurea-sensitive KATP channels on nerve terminals of the rat motor cortex

Abstract

Keywords

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