Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

David Lleres, Marco Denegri, Marco Biggiogera, Paul Ajuh, Angus I. Lamond

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    39 Citations (Scopus)

    Abstract

    Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

    Original languageEnglish
    Pages (from-to)445-451
    Number of pages7
    JournalEMBO Reports
    Volume11
    Issue number6
    DOIs
    Publication statusPublished - Jun 2010

    Keywords

    • alternative pre-mRNA splicing
    • FLIM-FRET
    • CDC5L
    • hnRNP-M
    • spliceosome
    • MESSENGER-RNA
    • IN-VIVO
    • RIBONUCLEOPROTEIN-M
    • HEAT-SHOCK
    • IDENTIFICATION
    • COMPLEX
    • SUBSET
    • BODIES
    • CELLS
    • CDC5L

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