Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

David Lleres; Marco Denegri; Marco Biggiogera; Paul Ajuh; Angus I. Lamond

doi:10.1038/embor.2010.64

Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

David Lleres, Marco Denegri, Marco Biggiogera, Paul Ajuh, Angus I. Lamond

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

Original language	English
Pages (from-to)	445-451
Number of pages	7
Journal	EMBO Reports
Volume	11
Issue number	6
DOIs	https://doi.org/10.1038/embor.2010.64
Publication status	Published - Jun 2010

Keywords

alternative pre-mRNA splicing
FLIM-FRET
CDC5L
hnRNP-M
spliceosome
MESSENGER-RNA
IN-VIVO
RIBONUCLEOPROTEIN-M
HEAT-SHOCK
IDENTIFICATION
COMPLEX
SUBSET
BODIES
CELLS
CDC5L

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title = "Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice",

abstract = "Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.",

keywords = "alternative pre-mRNA splicing, FLIM-FRET, CDC5L, hnRNP-M, spliceosome, MESSENGER-RNA, IN-VIVO, RIBONUCLEOPROTEIN-M, HEAT-SHOCK, IDENTIFICATION, COMPLEX, SUBSET, BODIES, CELLS, CDC5L",

author = "David Lleres and Marco Denegri and Marco Biggiogera and Paul Ajuh and Lamond, {Angus I.}",

year = "2010",

month = jun,

doi = "10.1038/embor.2010.64",

language = "English",

volume = "11",

pages = "445--451",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "EMBO Press",

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TY - JOUR

T1 - Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

AU - Lleres, David

AU - Denegri, Marco

AU - Biggiogera, Marco

AU - Ajuh, Paul

AU - Lamond, Angus I.

PY - 2010/6

Y1 - 2010/6

N2 - Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

AB - Heterogeneous nuclear ribonucleoprotein-M (hnRNP-M) is an abundant nuclear protein that binds to pre-mRNA and is a component of the spliceosome complex. A direct interaction was detected in vivo between hnRNP-M and the human spliceosome proteins cell division cycle 5-like (CDC5L) and pleiotropic regulator 1 (PLRG1) that was inhibited during the heat-shock stress response. A central region in hnRNP-M is required for interaction with CDC5L/PLRG1. hnRNP-M affects both 50 and 30 alternative splice site choices, and an hnRNP-M mutant lacking the CDC5L/PLRG1 interaction domain is unable to modulate alternative splicing of an adeno-E1A mini-gene substrate.

KW - alternative pre-mRNA splicing

KW - FLIM-FRET

KW - CDC5L

KW - hnRNP-M

KW - spliceosome

KW - MESSENGER-RNA

KW - IN-VIVO

KW - RIBONUCLEOPROTEIN-M

KW - HEAT-SHOCK

KW - IDENTIFICATION

KW - COMPLEX

KW - SUBSET

KW - BODIES

KW - CELLS

KW - CDC5L

U2 - 10.1038/embor.2010.64

DO - 10.1038/embor.2010.64

M3 - Article

C2 - 20467437

SN - 1469-221X

VL - 11

SP - 445

EP - 451

JO - EMBO Reports

JF - EMBO Reports

IS - 6

ER -

Direct interaction between hnRNP-M and CDC5L/PLRG1 proteins affects alternative splice site choice

Abstract

Keywords

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