Direct measurement of inositol in bovine myelin basic protein

Ross Smith, Peter E. Braun, Michael A. J. Ferguson, Martin G. Low, William R. Sherman

    Research output: Contribution to journalArticle

    10 Citations (Scopus)

    Abstract

    Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.
    Original languageEnglish
    Pages (from-to)285-288
    Number of pages4
    JournalBiochemical Journal
    Volume248
    Issue number1
    Publication statusPublished - 15 Nov 1987

    Cite this

    Smith, R., Braun, P. E., Ferguson, M. A. J., Low, M. G., & Sherman, W. R. (1987). Direct measurement of inositol in bovine myelin basic protein. Biochemical Journal, 248(1), 285-288.
    Smith, Ross ; Braun, Peter E. ; Ferguson, Michael A. J. ; Low, Martin G. ; Sherman, William R. / Direct measurement of inositol in bovine myelin basic protein. In: Biochemical Journal. 1987 ; Vol. 248, No. 1. pp. 285-288.
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    abstract = "Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol {\%} of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.",
    author = "Ross Smith and Braun, {Peter E.} and Ferguson, {Michael A. J.} and Low, {Martin G.} and Sherman, {William R.}",
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    Smith, R, Braun, PE, Ferguson, MAJ, Low, MG & Sherman, WR 1987, 'Direct measurement of inositol in bovine myelin basic protein', Biochemical Journal, vol. 248, no. 1, pp. 285-288.

    Direct measurement of inositol in bovine myelin basic protein. / Smith, Ross; Braun, Peter E.; Ferguson, Michael A. J.; Low, Martin G.; Sherman, William R.

    In: Biochemical Journal, Vol. 248, No. 1, 15.11.1987, p. 285-288.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Direct measurement of inositol in bovine myelin basic protein

    AU - Smith, Ross

    AU - Braun, Peter E.

    AU - Ferguson, Michael A. J.

    AU - Low, Martin G.

    AU - Sherman, William R.

    PY - 1987/11/15

    Y1 - 1987/11/15

    N2 - Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

    AB - Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

    M3 - Article

    VL - 248

    SP - 285

    EP - 288

    JO - Biochemical Journal

    JF - Biochemical Journal

    SN - 0264-6021

    IS - 1

    ER -

    Smith R, Braun PE, Ferguson MAJ, Low MG, Sherman WR. Direct measurement of inositol in bovine myelin basic protein. Biochemical Journal. 1987 Nov 15;248(1):285-288.