Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.
|Number of pages||4|
|Publication status||Published - 15 Nov 1987|
Smith, R., Braun, P. E., Ferguson, M. A. J., Low, M. G., & Sherman, W. R. (1987). Direct measurement of inositol in bovine myelin basic protein. Biochemical Journal, 248(1), 285-288. http://www.biochemj.org/bj/248/bj2480285.htm